Summary of Study ST000253
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000205. The data can be accessed directly via it's Project DOI: 10.21228/M8WK5Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000253 |
Study Title | NIH WCMC Pilot & Feasibility Project: “Metabolomics of Neonatal Pulmonary Hypertension” |
Study Type | Treatment and feeding study |
Study Summary | Targeted metabolomic analyses of oxylipins were performed on 16 rat plasma and lung samples collected from rats euthanized at 14 days following exposure to growth restriction and/or hyperoxia. Samples were analyzed by UPLC-MS/MS using a Waters Acquity UPLC and detected on an API 4000 QTrap (AB Sciex, Framingham, MA, USA) by multiple reaction monitoring (MRM) after negative mode electrospray ionization. |
Institute | University of California, Davis |
Department | U.S.D.A. Western Human Nutrition Research Center |
Laboratory | Newman Lab |
Last Name | Newman |
First Name | John |
Address | 430 W. Health Sciences Dr., Davis, CA 95616 |
john.newman@ars.usda.gov | |
Phone | +1-530-752-1009 |
Submit Date | 2015-09-03 |
Num Groups | 4 |
Total Subjects | 16 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Uploaded File Size | 16 M |
Analysis Type Detail | LC-MS |
Release Date | 2015-09-14 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000400 | AN000401 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | ||
Chromatography system | Waters Acquity | Waters Acquity |
Column | ||
MS Type | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole |
MS instrument name | ABI Sciex API 4000 QTrap | ABI Sciex API 4000 QTrap |
Ion Mode | NEGATIVE | NEGATIVE |
Units | % Area Ratio | nM |
Chromatography:
Chromatography ID: | CH000283 |
Chromatography Summary: | Oxylipin analysis |
Methods Filename: | NewmanLab-F&BOxys-Protocol-PT.pdf |
Instrument Name: | Waters Acquity |
Column Temperature: | 60 C |
Flow Gradient: | See protocol/methods file |
Flow Rate: | 0.25 |
Internal Standard: | See protocol/methods file |
Retention Time: | See protocol/methods file |
Sample Injection: | 5 L |
Solvent A: | 100% water; 0.1% acetic acid |
Solvent B: | 90% acetonitrile/ 10% isopropanol |
Analytical Time: | 16 min |
Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
Weak Wash Volume: | 600 L |
Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
Strong Wash Volume: | 600 L |
Sample Loop Size: | 17 L |