Summary of Study ST000259

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000210. The data can be accessed directly via it's Project DOI: 10.21228/M87W2S This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST000259
Study TitleMetabolic contribution of pSymA and pSymB megaplasmid/chromid for multipartite Sinorhizobium meliloti cultured in rich LBmc medium
Study Typemegaplasmid deletion
Study SummaryWe wished to evaluate the contribution of pSymA and pSymB towards the utilization of various metabolites in a nutritionally complex environment and to examine how S. meliloti influences its surrounding environment.
McMaster University
DepartmentDepartment of Biology
Last NameFinan
First NameTurlough
AddressDepartment of Biology, McMaster University, Hamilton, Canada L8S4K1
Phone(+1)905-525-9140 ext 22932
Submit Date2015-09-17
Num Groups14
Total Subjects82
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2015-10-26
Release Version1
Turlough Finan Turlough Finan application/zip

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN000411 AN000412
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Agilent 1200 RR Series II Agilent 1200 RR Series II
Column Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um)
MS instrument type QTOF QTOF
MS instrument name Bruker MicrOTOF II Bruker MicrOTOF II
Units Normalized Peak area Normalized Peak area


Chromatography ID:CH000291
Methods Filename:Opt_silicaHILIC.m
Instrument Name:Agilent 1200 RR Series II
Column Name:Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um)
Column Temperature:40C
Flow Gradient:0-0.5 min, 95%A; 0.5-12.5 min, 35%A; 12.5-13.0 min, 35%A; 13.0-14.0 min, 95%A; 14.0-24.0 min, 95%A
Flow Rate:200 ΔL/min
Internal Standard:L-phenylalanine-d8, diphenylalanine and glycine-phenylalanine
Sample Injection:2 ΔL
Solvent A:100% acetonitrile
Solvent B:100% water;10 mM ammonium acetate, adjust to pH 3 with formic acid
Analytical Time:24 min
Chromatography Type:HILIC