Summary of Study ST000259
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000210. The data can be accessed directly via it's Project DOI: 10.21228/M87W2S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000259 |
Study Title | Metabolic contribution of pSymA and pSymB megaplasmid/chromid for multipartite Sinorhizobium meliloti cultured in rich LBmc medium |
Study Type | megaplasmid deletion |
Study Summary | We wished to evaluate the contribution of pSymA and pSymB towards the utilization of various metabolites in a nutritionally complex environment and to examine how S. meliloti influences its surrounding environment. |
Institute | McMaster University |
Department | Department of Biology |
Last Name | Finan |
First Name | Turlough |
Address | Department of Biology, McMaster University, Hamilton, Canada L8S4K1 |
finan@mcmaster.ca | |
Phone | (+1)905-525-9140 ext 22932 |
Submit Date | 2015-09-17 |
Num Groups | 14 |
Total Subjects | 82 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2015-10-26 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000411 | AN000412 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Agilent 1200 RR Series II | Agilent 1200 RR Series II |
Column | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Bruker MicrOTOF II | Bruker MicrOTOF II |
Ion Mode | POSITIVE | NEGATIVE |
Units | Normalized Peak area | Normalized Peak area |
Chromatography:
Chromatography ID: | CH000291 |
Methods Filename: | Opt_silicaHILIC.m |
Instrument Name: | Agilent 1200 RR Series II |
Column Name: | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
Column Temperature: | 40C |
Flow Gradient: | 0-0.5 min, 95%A; 0.5-12.5 min, 35%A; 12.5-13.0 min, 35%A; 13.0-14.0 min, 95%A; 14.0-24.0 min, 95%A |
Flow Rate: | 200 ΔL/min |
Internal Standard: | L-phenylalanine-d8, diphenylalanine and glycine-phenylalanine |
Sample Injection: | 2 ΔL |
Solvent A: | 100% acetonitrile |
Solvent B: | 100% water;10 mM ammonium acetate, adjust to pH 3 with formic acid |
Analytical Time: | 24 min |
Chromatography Type: | HILIC |