Summary of Study ST000601
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000438. The data can be accessed directly via it's Project DOI: 10.21228/M8FC7C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000601 |
Study Title | Untargeted LC-MS metabolomics analysis of human COPD plasma, HILIC & C18 |
Study Summary | Identify perturbed metabolites and pathways in human plasma collected from 131 COPD subjects. Subjects were either current or former smokers with various COPD phenotypes including emphysema, and exacerbations. |
Institute | University of Colorado Denver |
Department | Anschutz Medical Campus, Pharmaceutical Sciences |
Last Name | Reisdorph |
First Name | Nichole |
Address | 12850 East Montview Blvd, Aurora, CO 80045 |
Nichole.Reisdorph@ucdenver.edu | |
Phone | 303-724-9243 |
Submit Date | 2017-04-27 |
Total Subjects | 131 |
Num Males | 56 |
Num Females | 75 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2018-10-10 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000920 | AN000921 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | HILIC |
Chromatography system | Agilent 6210 | Agilent 6520 |
Column | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
MS Type | ESI | ESI |
MS instrument type | TOF | QTOF |
MS instrument name | Agilent | Agilent |
Ion Mode | POSITIVE | POSITIVE |
Units | Count | Counts |
Chromatography:
Chromatography ID: | CH000656 |
Chromatography Summary: | Lipid Fraction (C18) |
Instrument Name: | Agilent 6210 |
Column Name: | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) |
Column Temperature: | 60°C |
Flow Gradient: | 0-0.5 minutes 30-70% B, 0.5-7.42 minutes 70-100% B, 7.42-9.9 minutes 100% B, 9.9-10.0 minutes 100-30% B, followed by column re-equilibration |
Flow Rate: | 0.7 mL/min |
Injection Temperature: | 4°C |
Sample Injection: | 4µL |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 60% isopropanol/36% acetonitrile/4% water; 0.1% formic acid |
Capillary Voltage: | 4000V |
Randomization Order: | Excel rand() function |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000657 |
Chromatography Summary: | Aqueous Fraction (HILIC) |
Instrument Name: | Agilent 6520 |
Column Name: | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
Column Temperature: | 20°C |
Flow Gradient: | 0.2 minutes 100% B, 2-2.1 minutes 100-90% B, 2.1-8.6 minutes 90-50% B, 8.6-8.7 minutes 50-0% B, 8.7-14.7 minutes 0% B, 14.7-14.8 minutes 0-100% B, followed by column re-equilibration |
Flow Rate: | 0.6 mL/min |
Injection Temperature: | 4°C |
Sample Injection: | 1µL |
Solvent A: | 50% acetonitrile/50% water; ammonium acetate, pH 5.8 |
Solvent B: | 90% acetonitrile/10% water; pH 5.8 ammonium acetate |
Capillary Voltage: | 4000V |
Randomization Order: | Excel rand() function |
Chromatography Type: | HILIC |