Summary of Study ST000601
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000438. The data can be accessed directly via it's Project DOI: 10.21228/M8FC7C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000601 |
Study Title | Untargeted LC-MS metabolomics analysis of human COPD plasma, HILIC & C18 |
Study Summary | Identify perturbed metabolites and pathways in human plasma collected from 131 COPD subjects. Subjects were either current or former smokers with various COPD phenotypes including emphysema, and exacerbations. |
Institute | University of Colorado Denver |
Department | Anschutz Medical Campus, Pharmaceutical Sciences |
Last Name | Reisdorph |
First Name | Nichole |
Address | 12850 East Montview Blvd, Aurora, CO 80045 |
Nichole.Reisdorph@ucdenver.edu | |
Phone | 303-724-9243 |
Submit Date | 2017-04-27 |
Total Subjects | 131 |
Num Males | 56 |
Num Females | 75 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2018-10-10 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000631 |
Sampleprep Summary: | 100µL of plasma was prepared using methanol to extract precipitate the proteins into a pellet. A liquid-liquid extraction with water and methyl-tert butyl ether (MTBE) was used to partition the supernatant into an aqueous layer and an organic layer (lipids). The aqueous layer was dried down in a SpeedVac at 45°C, then reconstituted in 100µL of 95:5 water:acetonitrile. The lipid fraction was dried down in a SpeedVac at 45°C, then reconstituted in 200µL of 100% methanol. |