Summary of Study ST001724
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001104. The data can be accessed directly via it's Project DOI: 10.21228/M80M6C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001724 |
| Study Title | The effects of birth weight and breeding value for protein deposition on the urine metabolome in growing pigs (part-III) |
| Study Summary | An experiment was conducted with growing pigs, to determine the effects of birth weight (BiW) and estimated breeding value for protein deposition (EBV) on the metabolomic profile in urine samples collected under different dietary regimens: protein adequate (A) or protein restricted (R, 70% of A). |
| Institute | Aarhus University |
| Department | Animal Science |
| Laboratory | Metabolomics LC-MS platform Aarhus University Foulum |
| Last Name | Hedemann |
| First Name | Mette |
| Address | Blichers Alle 20, Tjele, -, 8830, Denmark |
| Mette.Hedemann@anis.au.dk | |
| Phone | 51448783 |
| Submit Date | 2021-03-15 |
| Total Subjects | 40 |
| Num Males | 40 |
| Publications | The effects of birth weight and breeding value for protein deposition on nitrogen efficiency in growing pigs |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-03-31 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002808 | AN002809 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
| Column | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Bruker Impact HD | Bruker Impact HD |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH002075 |
| Chromatography Summary: | Chromatographic separation was performed on a Dionex UltiMate 3000RSL Binary UHPLC System (Thermo Scientific Dionex, Sunnyvale, CA) equipped with a HSS T3 C18 UHPLC column, 1.8 µm, 100x2.1 mm (Waters Corporation, Milford, MA). The column was maintained at 30°C. Samples were kept in the autosampler at 10°C, and the injection volume was 3 µl. The mobile phases were 0.1% formic acid in Milli-Q water (A) and 0.1% formic acid in acetonitrile (B). The gradient program for the urine samples was as follows: 0-8 min, linear gradient from 5-70% B; 8-8.5 min, linear gradient from 70-100% B; 8.5-9.5 min, 100% B and return to initial conditions in 0.2 min. The column was re-equilibrated at 5% B for 2 min at the beginning of each run and the flow rate was set to 400 µL/min. |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 30 |
| Flow Rate: | 0.4 ml/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
