Summary of Study ST002066
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001308. The data can be accessed directly via it's Project DOI: 10.21228/M8N98X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002066 |
Study Title | Glutaminase inhibition impairs CD8 T cell activation in STK11/Lkb1 deficient lung cancer |
Study Type | Biomedical research |
Study Summary | To characterize the impact of KRAS co-mutations KEAP1 and STK11/Lkb1 on the metabolic and immune microenvironment of lung adenocarcinoma in immune-intact models, we generated a cohort of genetically engineered mouse models (GEMMs) to reflect the diverse tumor suppressor landscape seen in patients. Mice carrying the KrasG12D allele (K)21 were crossed with Keap1flox/flox (KK)22 and/or Lkb1flox/flox (KKL, KL)23 mice and genetic recombination induced by intranasal inhalation of Ad5-CMV-Cre adenovirus. We interrogated the metabolites present in lung tumor nodules collected from cohorts of KK, KL and KKL mice. |
Institute | The Walter and Eliza Hall Institute of Medical Research |
Laboratory | Kate Sutherland |
Last Name | Sarah |
First Name | Best |
Address | 1G, Royal Parade, Parkville VIC 3052, Australia |
best@wehi.edu.au | |
Phone | +61-3-9345-2452 |
Submit Date | 2022-01-18 |
Num Groups | 5 |
Total Subjects | 25 |
Num Males | 17 |
Num Females | 8 |
Study Comments | L lobe of mice lung |
Publications | Glutaminase inhibition impairs CD8 T cell activation in STK11/Lkb1 deficient lung cancer |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2022-05-02 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003365 | AN003366 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
Column | SeQuant ZIC-pHILIC (150 x 4.2mm,5um) | SeQuant ZIC-pHILIC (150 x 4.2mm,5um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units |
Chromatography:
Chromatography ID: | CH002489 |
Chromatography Summary: | LCMS data was acquired on Q-Exactive Orbitrap mass spectrometer (Thermo Fisher) coupled with high-performance liquid chromatography (HPLC) system Dionex Ultimate® 3000 RS (Thermo Fisher). Chromatographic separation was performed on a ZIC-pHILIC column (5 µm, polymeric, 150 × 4.6 mm, SeQuant®, Merck). The mobile phase (A) was 20 mM ammonium carbonate and (B) acetonitrile. The gradient program started at 80% B and was reduced to 50% B over 15 min, then reduced from 50% B to 5% B over 3 min, followed by wash with 5% B for another 3 min, and finally 8 min re-equilibration with 80% B. The flow rate was 0.3 mL/min and column compartment temperature was 40ºC. The total run time was 32 min with an injection sample volume of 10 µL. |
Methods Filename: | Metabolomics_pHILIC_Parkville_v1.meth |
Instrument Name: | Thermo Dionex Ultimate 3000 RS |
Column Name: | SeQuant ZIC-pHILIC (150 x 4.2mm,5um) |
Column Pressure: | 60 bar at starting conditions |
Column Temperature: | 25 C |
Flow Gradient: | 0 min - 80%B, 15 min - 50%B, 18 min - 5%B, 21 min - 5%B, 24 min - 80%B, 32 min - 80%B |
Flow Rate: | 0.3 ml/min |
Injection Temperature: | 4 C |
Internal Standard: | CAPS, CHAPS, PIPES |
Sample Injection: | 10 uL |
Solvent A: | 100% water; 20 mM ammonium formate |
Solvent B: | 100% acetonitrile |
Analytical Time: | 32 min |
Capillary Voltage: | 3.5 kV |
Oven Temperature: | 25 C |
Washing Buffer: | syringe wash 50% IPA |
Weak Wash Solvent Name: | 10% MeOH |
Strong Wash Solvent Name: | 10% MeOH |
Sample Loop Size: | 25 uL |
Sample Syringe Size: | 25 uL |
Chromatography Type: | HILIC |