Summary of Study ST002280

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001460. The data can be accessed directly via it's Project DOI: 10.21228/M80T4P This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002280
Study TitleOxidative phosphorylation selectively orchestrates tissue macrophage homeostasis
Study TypeObservational study
Study SummaryIn vitro studies associated oxidative phosphorylation (OXPHOS) with anti-inflammatory macrophages, while pro-inflammatory macrophages rely on glycolysis. However, the metabolic needs of macrophages in tissues (TMFs) to fulfil their homeostatic activities are incompletely understood. Here, we identified OXPHOS as highly discriminating process among TMFs from different tissues in homeostasis by analysis of RNAseq data, in both human and mouse. Impairing OXPHOS in TMFs via Tfam deletion differentially affected TMF populations. Tfam deletion resulted in reduction of alveolar macrophages (AMs) due to impaired lipid-handling capacity, leading to increased cholesterol content and cellular stress, causing cell cycle arrest in vivo. In obesity, Tfam depletion selectively ablated pro-inflammatory lipid-handling white adipose tissue macrophages (WAT-MFs), preventing insulin resistance and hepatosteatosis. Thus, OXPHOS, rather than glycolysis, distinguishes TMF populations and is critical for the maintenance of TMFs with a high lipid-handling activity, including pro-inflammatory WAT-MFs. This could provide a selective therapeutic targeting tool.
Spanish National Center for Cardiovascular Research (CNIC)
DepartmentNovel mechanisms of atherosclerosis
Last NameMastrangelo
First NameAnnalaura
AddressCalle de Melchor Fernández Almagro, 3, Centro Nacional de Investigaciones Cardiovasculares
Phone(+34) 914531200
Submit Date2022-09-01
Num Groups2
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailGC-MS
Release Date2022-09-22
Release Version1
Annalaura Mastrangelo Annalaura Mastrangelo application/zip

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Combined analysis:

Analysis ID AN003724
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890B
Column Agilent DB5-MS (30m x 0.25mm, 0.25um)
MS Type EI
MS instrument type QTOF
MS instrument name Agilent 7250 GC/Q-TOF
Units relative abundance


Chromatography ID:CH002758
Chromatography Summary:Derivative samples (2 μL) were injected into a GC column DB5–MS (30 m length, 0.250 mm i.d., 0.25 μm film 95% dimethyl/5% diphenylpolysiloxane) with a pre–column (10 m J&W integrated with Agilent 122–5532G). The temperature gradient was programmed at 60 °C (held for 1 min), with a ramping increase rate of 10 °C/min up to 325°C (held for 10 min). The total analysis time was 37.5 min. Two analytical replicates for each sample were injected.
Methods Filename:Protocol_AM.pdf
Instrument Name:Agilent 7890B
Column Name:Agilent DB5-MS (30m x 0.25mm, 0.25um)
Chromatography Type:GC