Summary of Study ST002280

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001460. The data can be accessed directly via it's Project DOI: 10.21228/M80T4P This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST002280
Study TitleOxidative phosphorylation selectively orchestrates tissue macrophage homeostasis
Study TypeObservational study
Study SummaryIn vitro studies associated oxidative phosphorylation (OXPHOS) with anti-inflammatory macrophages, while pro-inflammatory macrophages rely on glycolysis. However, the metabolic needs of macrophages in tissues (TMFs) to fulfil their homeostatic activities are incompletely understood. Here, we identified OXPHOS as highly discriminating process among TMFs from different tissues in homeostasis by analysis of RNAseq data, in both human and mouse. Impairing OXPHOS in TMFs via Tfam deletion differentially affected TMF populations. Tfam deletion resulted in reduction of alveolar macrophages (AMs) due to impaired lipid-handling capacity, leading to increased cholesterol content and cellular stress, causing cell cycle arrest in vivo. In obesity, Tfam depletion selectively ablated pro-inflammatory lipid-handling white adipose tissue macrophages (WAT-MFs), preventing insulin resistance and hepatosteatosis. Thus, OXPHOS, rather than glycolysis, distinguishes TMF populations and is critical for the maintenance of TMFs with a high lipid-handling activity, including pro-inflammatory WAT-MFs. This could provide a selective therapeutic targeting tool.
Spanish National Center for Cardiovascular Research (CNIC)
DepartmentNovel mechanisms of atherosclerosis
Last NameMastrangelo
First NameAnnalaura
AddressCalle de Melchor Fernández Almagro, 3, Centro Nacional de Investigaciones Cardiovasculares
Phone(+34) 914531200
Submit Date2022-09-01
Num Groups2
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailGC-MS
Release Date2022-09-22
Release Version1
Annalaura Mastrangelo Annalaura Mastrangelo application/zip

Select appropriate tab below to view additional metadata details:


Collection ID:CO002359
Collection Summary:Mouse colonies were bred at the CNIC under specific pathogen-free conditions and on C57BL/6 background. Tfamf/f (Larsson et al., 1998) mice were kindly provided by Nils-Göran Larsson (Max Planck Institute for Biology of Ageing, Cologne, Germany). All floxed mouse lines were crossed with CD11cCre mice (Caton et al., 2007). Mice were group-housed, have not been used in previous procedures and were fed standard chow. Littermates of the same sex were randomly assigned to experimental groups. Male and female mice were used for all experiments. Mice with 6–10-weeks (adult) were used for the experiment. Bronchoalveolar lavage (BAL) was performed by inserting a venal catheter (BD) into the trachea and 3-10 washes with 0.3-1 ml FACS buffer to harvest BAL cells.
Collection Protocol Filename:Protocol_AM.pdf
Sample Type:Bronchoalveolar lavage
Collection Method:Bronchoalveolar lavage (BAL) was performed by inserting a venal catheter (BD) into the trachea and 3-10 washes with 0.3-1 ml FACS buffer to harvest BAL cells.
Storage Conditions:-80℃