Summary of Study ST002571

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001658. The data can be accessed directly via it's Project DOI: 10.21228/M8CX2C This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002571
Study TitleQuantification of cytokinins in ap1 cal AP1-GR and drmy1 ap1 cal AP1-GR inflorescences using LC-MS
Study TypeQuantification using mass spectrometry
Study SummaryRobustness is the invariant development of phenotype despite environmental changes and genetic perturbations. In the Arabidopsis flower bud, four sepals initiate at robust positions and times and grow to equal size to enclose and protect the inner floral organs. We previously characterized the mutant development related myb-like1 (drmy1), where 3-5 sepals initiate at irregular positions and variable times and grow to different sizes, compromising their protective function. This loss of robustness was caused by a uniform increase in cytokinin signaling, as revealed by the TCS::GFP reporter, in the floral meristem before sepal initiation. We hypothesized that the increase in cytokinin signaling in drmy1 was due to an increase in the level of cytokinins. To test this idea, we extracted cytokinins from induced inflorescences of wild-type (5 bio-reps) and drmy1 (6 bio-reps) in ap1 cal AP1-GR background. We measured the level of three cytokinin bases, trans-Zeatin (tZ), cis-Zeatin (cZ), and isopentenyladenine (iP), and their corresponding nucleosides (tZR, cZR, and iPR), using liquid chromatography-mass spectrometry. We found that there was no statistically significant differences in cytokinin levels between these genotypes, indicating that the increase in cytokinin signaling in the drmy1 floral meristem is not due to increased cytokinin levels.
Cornell University
DepartmentPlant Biology Section
LaboratoryRoeder Lab
Last NameKong
First NameShuyao
Address239 Weill Hall, 526 Campus Road, Ithaca, NY 14853
Submit Date2023-04-20
Num Groups2
Total Subjects11
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-05-11
Release Version1
Shuyao Kong Shuyao Kong application/zip

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Combined analysis:

Analysis ID AN004236
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap
Units Peak area


Chromatography ID:CH003143
Chromatography Summary:1 µl of each sample was injected into a Thermo Fisher Scientific Vanquish Horizon UHPLC System coupled with a Thermo Q Exactive HF hybrid quadropole-orbitrap high-resolution mass spectrometer equipped with a HESI ion source. Samples were separated on a C18 ODS column (AQUITY UPLC BEH C18, 1.7 μm, 2.1 × 100 mm, Waters), at a flow rate of 0.3 ml/min, with linear gradients of solvent A (0.1% formic acid) and solvent B (0.1% formic acid in methanol) according to the following profile: 0 min, 99.0% A + 1.0% B; 4.0 min, 55.0% A + 45.0% B; 7 min, 30.0% A + 70.0% B; and then with isocratic conditions: 8 min, 1.0% A + 99.0% B; 12 min, 99.0% A + 1.0% B.
Methods Filename:Protocol_SK.PDF
Instrument Name:Waters Acquity
Column Name:Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
Column Temperature:60 °C
Flow Gradient:0 min, 99.0% A + 1.0% B; 4.0 min, 55.0% A + 45.0% B; 7 min, 30.0% A + 70.0% B; and then with isocratic conditions: 8 min, 1.0% A + 99.0% B; 12 min, 99.0% A + 1.0% B
Flow Rate:0.3 ml/min
Internal Standard:BAP
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Preconditioning:Mili-Q H2O
Chromatography Type:Reversed phase