Summary of Study ST002571

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001658. The data can be accessed directly via it's Project DOI: 10.21228/M8CX2C This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002571
Study TitleQuantification of cytokinins in ap1 cal AP1-GR and drmy1 ap1 cal AP1-GR inflorescences using LC-MS
Study TypeQuantification using mass spectrometry
Study SummaryRobustness is the invariant development of phenotype despite environmental changes and genetic perturbations. In the Arabidopsis flower bud, four sepals initiate at robust positions and times and grow to equal size to enclose and protect the inner floral organs. We previously characterized the mutant development related myb-like1 (drmy1), where 3-5 sepals initiate at irregular positions and variable times and grow to different sizes, compromising their protective function. This loss of robustness was caused by a uniform increase in cytokinin signaling, as revealed by the TCS::GFP reporter, in the floral meristem before sepal initiation. We hypothesized that the increase in cytokinin signaling in drmy1 was due to an increase in the level of cytokinins. To test this idea, we extracted cytokinins from induced inflorescences of wild-type (5 bio-reps) and drmy1 (6 bio-reps) in ap1 cal AP1-GR background. We measured the level of three cytokinin bases, trans-Zeatin (tZ), cis-Zeatin (cZ), and isopentenyladenine (iP), and their corresponding nucleosides (tZR, cZR, and iPR), using liquid chromatography-mass spectrometry. We found that there was no statistically significant differences in cytokinin levels between these genotypes, indicating that the increase in cytokinin signaling in the drmy1 floral meristem is not due to increased cytokinin levels.
Cornell University
DepartmentPlant Biology Section
LaboratoryRoeder Lab
Last NameKong
First NameShuyao
Address239 Weill Hall, 526 Campus Road, Ithaca, NY 14853
Submit Date2023-04-20
Num Groups2
Total Subjects11
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-05-11
Release Version1
Shuyao Kong Shuyao Kong application/zip

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Combined analysis:

Analysis ID AN004236
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap
Units Peak area


MS ID:MS003983
Analysis ID:AN004236
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Comments:Cytokinins were detected using the positive ion mode. For tZ, tZR, iP, iPR, and the internal control BAP, peaks were identified from an external standard mix composed of 0.1 µg/ml each of BAP (Alfa Aesar A14678), tZ (Sigma Z0876), tZR (Sigma Z3541), iP (Cayman Chemical 17906), and iPR (Cayman chemical 20522) in 5% acetonitrile. For cZ and cZR, peaks were identified based on previously reported precursor m/z and retention time. Using Xcalibur (Thermo Scientific), peak area was quantified for each cytokinin in each sample, normalized against the peak area of BAP (internal control) and sample fresh weight, and then normalized against the average abundance of tZ in WT samples.