Summary of Study ST002960
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001842. The data can be accessed directly via it's Project DOI: 10.21228/M8MH8P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002960 |
Study Title | Analysis of Lipids Secreted from Fibroblast Young Cells |
Study Summary | In this experimental study, we aimed to understand the potential factors within the secretions of young cells that could trigger the reverse aging of Mid-old cells. To investigate this phenomenon, we co-cultured young cells with Mid-old cells and observed a fascinating outcome: the Mid-old cells exhibited reverse aging and transformed into a more youthful state. To uncover the specific factors responsible for this reverse aging effect, we conducted a detailed analysis of the secreted factors from the young cells. Our analysis focused on a range of biomolecules, including lipids. However, despite our efforts, we did not identify any distinct factors that could be directly attributed to this remarkable reverse aging process. |
Institute | Ajou University Medical Center |
Last Name | Kim |
First Name | Young Hwa |
Address | 206, World cup-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea |
skyblue32@nate.com | |
Phone | +82-10-5153-3636 |
Submit Date | 2023-11-01 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2023-11-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004861 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1290 Infinity UHPLC |
Column | Thermo Hypersil GOLD C18 (100 x 2.1mm,1.9um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Q Exactive™ Hybrid Quadrupole-Orbitrap MS |
Ion Mode | POSITIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH003669 |
Methods Filename: | LC_MS_Method_lipid.pdf |
Instrument Name: | Agilent 1290 Infinity UHPLC |
Column Name: | Thermo Hypersil GOLD C18 (100 x 2.1mm,1.9um) |
Column Temperature: | 320℃ |
Flow Gradient: | 0–5 min, 5% B; 5–15 min, 5–30% B; 15–22 min, 30–90% B; 22–25 min, 90% B; 25–26 min, 90–5% B; 26–30 min, 5% B. Parameters were set as follows: positive mode, spray voltage; 3.8 kV |
Flow Rate: | 0.2 mL/min |
Solvent A: | Acetonitrile:Methanol:Water (19:19:2, v/v/v) + 20 mmol/L ammonium formate + 0.1% formic acid (v/v) |
Solvent B: | 2-propanol + 20 mmol/L ammonium formate + 0.1% formic acid (v/v) |
Chromatography Type: | Reversed phase |