Summary of Study ST001666
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001070. The data can be accessed directly via it's Project DOI: 10.21228/M8D12Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001666 |
| Study Title | Branched-chain alpha-ketoacids are preferentially reaminated and activate protein synthesis in the mouse heart |
| Study Summary | Branched-chain amino acids (BCAA) and their cognate α-ketoacids (BCKA) are elevated in an array of cardiometabolic diseases. Here we demonstrate that sequestration of BCAA and BCKA away from mitochondrial oxidation is likely due to low levels of expression of the mitochondrial BCAA transporter SLC25A44 in the heart, as its overexpression significantly lowers accumulation of [13C]-labeled valine from [U-13C]KIV. |
| Institute | Duke University |
| Last Name | Walejko |
| First Name | Jacquelyn |
| Address | 300 N Duke St |
| jacquelyn.walejko@duke.edu | |
| Phone | 9194792304 |
| Submit Date | 2021-01-26 |
| Analysis Type Detail | GC-MS |
| Release Date | 2021-02-09 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO001736 |
| Collection Summary: | Fed male C57BL/6J mice were anesthetized with 5% isoflurane, and isolated hearts were perfused in the Langendorff mode at 37°C with non-recirculating perfusate. The hearts were allowed to beat spontaneously throughout the perfusion. At the end of each perfusion, hearts were immediately freeze-clamped in liquid nitrogen using the Wollenberger technique and stored at -80 oC for further analysis. |
| Sample Type: | Heart |
