Summary of Study ST001818
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001149. The data can be accessed directly via it's Project DOI: 10.21228/M8611G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001818 |
| Study Title | Quantification of PIPs species in mouse embryonic fibroblasts (MEFs) during autophagosome formation. |
| Study Summary | Using a supercritical fluid chromatography-mass spectrometry (SFC-MS)-based methodology, we quantified phosphoinositides (PIPs) species in mouse embryonic fibroblasts (MEFs) from WT or FIP200 KO mice during autophagosome formation. |
| Institute | Grad Sch of Pharmaceut Sci, Univ of Tokyo |
| Last Name | Kono |
| First Name | Nozomu |
| Address | Hongo 7-3-1,, Bunyo-ku, Tokyo, 113-0033, Japan |
| nozomu@mol.f.u-tokyo.ac.jp | |
| Phone | +81-3-5841-4723 |
| Submit Date | 2021-06-02 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-05-04 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO001888 |
| Collection Summary: | MEFs were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich) supplemented with 10% fetal calf serum, 100 units/ml penicillin, 100 µg/ml streptomycin, and 2 mM L-glutamine. For autophagy induction, 2 × 105 cells were seeded on 6-well plates and the next day, cells were washed twice with PBS and incubated in amino acid-free EBSS (starvation medium; Thermo Fisher Scientific 24010-043) in the presence or absence of bafilomycin A1 (Selleck Chemicals) for 30 min at 37 ºC. After the incubation, cells were washed twice with PBS and collected to a safe-lock poly-propylene tube (2 ml) with 1 M HCl (500 μl), followed by centrifugation (15,000 g, 5 min at 4 ºC). Supernatants were removed rapidly and resuspended with 750 μl of quench mix, 170 μl of H2O. |
| Sample Type: | Fibroblasts |
