Summary of Study ST001818

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001149. The data can be accessed directly via it's Project DOI: 10.21228/M8611G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001818
Study TitleQuantification of PIPs species in mouse embryonic fibroblasts (MEFs) during autophagosome formation.
Study SummaryUsing a supercritical fluid chromatography-mass spectrometry (SFC-MS)-based methodology, we quantified phosphoinositides (PIPs) species in mouse embryonic fibroblasts (MEFs) from WT or FIP200 KO mice during autophagosome formation.
Institute
Grad Sch of Pharmaceut Sci, Univ of Tokyo
Last NameKono
First NameNozomu
AddressHongo 7-3-1,, Bunyo-ku, Tokyo, 113-0033, Japan
Emailnozomu@mol.f.u-tokyo.ac.jp
Phone+81-3-5841-4723
Submit Date2021-06-02
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2022-05-04
Release Version1
Nozomu Kono Nozomu Kono
https://dx.doi.org/10.21228/M8611G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002950
Analysis type MS
Chromatography type Unspecified
Chromatography system Shimadzu Nexera UC
Column ULTRON AF-HILIC-CD (250 × 2.1 mm,5.0um) Shinwa Chemicalstries
MS Type ESI
MS instrument type Ion trap
MS instrument name ABI SCIEX 4500 QTrap
Ion Mode POSITIVE
Units PI3P / 36:1PS

MS:

MS ID:MS002740
Analysis ID:AN002950
Instrument Name:ABI SCIEX 4500 QTrap
Instrument Type:Ion trap
MS Type:ESI
MS Comments:The instrument parameters of QTRAP4500 for positive ion mode were as follows: curtain gas, 20 psi; ionspray voltage, 4500 V; temperature, 300 ºC; ion source gas 1, 18 psi; ion source gas 2, 20 psi. and quantification was performed using MultiQuant™ software (SCIEX).
Ion Mode:POSITIVE
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