Summary of Study ST001912
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001206. The data can be accessed directly via it's Project DOI: 10.21228/M8TD6R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001912 |
| Study Title | Identification of effector metabolites using exometabolite profiling of diverse microalgae |
| Study Summary | We conducted an untargeted metabolomic analysis of non-polar exometabolites from four phylogenetically and ecologically diverse eukaryotic microalgal strains grown in the laboratory: freshwater Chlamydomonas reinhardtii, brackish Desmodesmus sp., marine Phaeodactylum tricornutum, and marine Microchloropsis salina. We analyzed both exometabolomes and cell pellet metabolomes to identify released metabolites based on relative enrichment in the exometabolomes. |
| Institute | Lawrence Berkeley National Laboratory |
| Last Name | Brisson |
| First Name | Vanessa |
| Address | 7000 East Ave, Livermore, CA, 94550, USA |
| brisson2@llnl.gov | |
| Phone | 5107177560 |
| Submit Date | 2021-08-23 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-09-10 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO001983 |
| Collection Summary: | Axenic algal were grown in glass 125 mL Erlenmeyer flasks that had previously been baked in a muffle furnace at 550°C for two hours to remove trace organics that could contaminate metabolomics analyses. Each flask contained 50 mL of the appropriate medium (defined freshwater or saltwater medium) and was capped with a foam stopper and covered with aluminum foil to avoid contamination during the experiment. Flasks were incubated with shaking at 90 rpm in a light incubator, with a 12-hour day/night light cycle. Daytime illumination was 3500 lux, and the incubator temperature was maintained at 22°C. Algal inocula were prepared by first growing algal stock cultures under experimental conditions (flasks, media, incubation) for one week to acclimate cultures to those conditions. Experimental flasks were inoculated by transferring 2 mL of inoculum culture to flasks with the appropriate medium. Cultures were harvested for metabolomic analysis from five replicate flasks for each alga after 8 days of incubation. Spent medium and cell pellets were separated by centrifugation. Half of the culture was transferred to a 50 mL falcon tube and centrifuged at 5000 g for eight minutes at 4°C. The supernatant was carefully transferred to a clean Falcon tube, and the remainder of the culture was added to the tube containing the pellet and the centrifugation procedure was repeated. After separation by centrifugation, the supernatant was filtered through a 0.45µm pore size syringe filter to remove residual algal cells. Filtered spent medium and cell pellets were frozen on dry ice and stored at -80°C until extraction. |
| Sample Type: | Algae |
| Storage Conditions: | -80℃ |
