Summary of Study ST001985
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001261. The data can be accessed directly via it's Project DOI: 10.21228/M8QD8K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001985 |
Study Title | Profiling Plasmodium falciparum parasites and human red blood cells after treatment with MMV693183 |
Study Summary | Compound MMV693183 was added to either Plasmodium falciparum or uninfected red blood cells. The concentrations used were 240 nM, 24 nM, and 2.4 nM. Untreated parasites and red blood cells were also used as controls. |
Institute | Pennsylvania State University |
Last Name | Llinás |
First Name | Manuel |
Address | W126 Millennium Science Complex, University Park, PENNSYLVANIA, 16802, USA |
manuel@psu.edu | |
Phone | 8148673527 |
Submit Date | 2021-10-26 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2022-02-16 |
Release Version | 1 |
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Collection:
Collection ID: | CO002059 |
Collection Summary: | Parasites were magnetically purified at the early trophozoite stage from cultures of 5-10% parasitemia. Samples were generated with 5 mL of media containing 1*10^8 cells (either parasites or human red blood cells) for the treatment condition. Excess media was removed and the cells were centrifuged and washed once with PBS. Metabolism was quenched with 90% methanol containing 0.25 micromolar of universally labeled C-13, N-15 aspartate. The samples were centrifuged and the metabolites in the methanol were collected and transferred to a new tube to be dried with nitrogen. |
Collection Protocol Filename: | Metabolite_Extraction_for_LCMS_2017.pdf |
Sample Type: | Cultured cells |
Collection Location: | Millenium Science Complex, University Park, Pennsylvania |
Storage Conditions: | -80℃ |
Collection Vials: | 1.5 mL eppendorf |
Storage Vials: | 1.5 mL eppendorf |
Collection Tube Temp: | On ice |
Tissue Cell Quantity Taken: | 1x10^8 cells per sample in 1 mL total volume |