Summary of Study ST000353

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000282. The data can be accessed directly via it's Project DOI: 10.21228/M8G308 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000353
Study TitleThe Development of Metabolomic Markers in African Bermudagrass (C. transvaalensis) for Sting Nematode (Belonolaimus longicaudatus) Response
Study TypeDisease response in terms of nematode reproduction and root weight
Study SummaryThe objective of the proposed pilot study is to identify metabolites up- and down-regulated in African bermudagrass that are tolerant and sensitive to the sting nematode and develop metabolomic markers for the highest expressed metabolites associated with tolerance. Future work will include additional accessions and species of bermudagrass, and testing under field conditions. Bermudagrass accessions identified as tolerant or sensitive by Pang et al. (2011) will be assessed under controlled greenhouse conditions to identify metabolites linked to sting nematode tolerance. Nematode response will be quantified through determination of root length and weight and the number of nematodes present 136 days after inoculation. Higher root length and weight indicate tolerance or resistance. Higher nematode counts indicate greater reproduction (i.e. a susceptible plant), while lower counts indicate that the accession may have some resistance. Metabolites from root tissue of these accessions will be compared to identify those associated with tolerance/resistance, and those that are associated with nematode infestation by comparing inoculated plants to uninoculated controls. Metabolomic markers will then be developed for the metabolites associated with tolerance/resistance. These markers will be used to guide future screening of bermudagrass accessions for breeding nematode-tolerant or -resistant varieties.
Institute
University of Florida
DepartmentSECIM
LaboratoryTurfgrass Breeding
Last NameBenda
First NameNicole
Address2005 SW 23rd St
Emailnbenda@ufl.edu
Phone352-792-4561
Submit Date2015-11-13
Num Groups3
Total Subjects42
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2017-07-10
Release Version1
Nicole Benda Nicole Benda
https://dx.doi.org/10.21228/M8G308
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Plant; Subject species: Cynodon transvaalensis (Factor headings shown in green)

mb_sample_id local_sample_id Plant genotype Nematode inoculation
SA015451AB03_I_13AB03 inoc
SA015452AB03_I_1AB03 inoc
SA015453AB03_I_10AB03 inoc
SA015454AB03_I_7AB03 inoc
SA015455AB03_I_2AB03 inoc
SA015456AB03_I_4AB03 inoc
SA015457AB03_I_5AB03 inoc
SA015458AB03_U_14AB03 uninoc
SA015459AB03_U_8AB03 uninoc
SA015460AB03_U_11AB03 uninoc
SA015461AB03_U_7AB03 uninoc
SA015462AB03_U_6AB03 uninoc
SA015463AB03_U_4AB03 uninoc
SA015464AB03_U_3AB03 uninoc
SA015465AB33_I_10AB33 inoc
SA015466AB33_I_11AB33 inoc
SA015467AB33_I_8AB33 inoc
SA015468AB33_I_5AB33 inoc
SA015469AB33_I_3AB33 inoc
SA015470AB33_I_1AB33 inoc
SA015471AB33_I_2AB33 inoc
SA015472AB33_U_11AB33 uninoc
SA015473AB33_U_9AB33 uninoc
SA015474AB33_U_13AB33 uninoc
SA015475AB33_U_2AB33 uninoc
SA015476AB33_U_8AB33 uninoc
SA015477AB33_U_4AB33 uninoc
SA015478AB33_U_7AB33 uninoc
SA015479AB39_I_11AB39 inoc
SA015480AB39_I_9AB39 inoc
SA015481AB39_I_12AB39 inoc
SA015482AB39_I_8AB39 inoc
SA015483AB39_I_3AB39 inoc
SA015484AB39_I_1AB39 inoc
SA015485AB39_I_4AB39 inoc
SA015486AB39_U_9AB39 uninoc
SA015487AB39_U_10AB39 uninoc
SA015488AB39_U_7AB39 uninoc
SA015489AB39_U_5AB39 uninoc
SA015490AB39_U_2AB39 uninoc
SA015491AB39_U_3AB39 uninoc
SA015492AB39_U_6AB39 uninoc
Showing results 1 to 42 of 42
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