Summary of Study ST000616

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000450. The data can be accessed directly via it's Project DOI: 10.21228/M8WC8S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000616
Study TitleHuman Aqueous Humor Phospholipids in Control and Glaucomatous eyes
Study SummaryAn analysis of phospholipid (phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine and phosphatidylinositol) classes in human control and glaucomatous aqueous humor (AH). Lipid extraction was performed using a modification of the Bligh and Dyer method, protein concentrations were determined using the Bradford’s method, and select samples were confirmed with Densitometry of PHAST gels. Lipids were identified and subjected to ratiometric quantification using a TSQ Quantum Access Max triple quadrupole mass spectrometer utilizing precursor ion scan (PIS) or neutral ion loss scan (NLS) using appropriate class specific lipid standards in a two-step quantification process. Mass spectrometer data were analyzed using MzMine 2.23.
Institute
University of Miami
Last NameBhattacharya
First NameSanjoy
Address1638 NW 10th Avenue, Miami, Florida, 33136, USA
Emailsbhattacharya@med.miami.edu
Phone3054824103
Submit Date2017-05-31
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailMS(Dir. Inf.)
Release Date2019-07-17
Release Version1
Sanjoy Bhattacharya Sanjoy Bhattacharya
https://dx.doi.org/10.21228/M8WC8S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Condition
SA034156NAH16L1Control
SA034157NAH14L1Control
SA034158NAH13L1Control
SA034159NAH17L1Control
SA034160NAH19L1Control
SA034161NAH1BL1Control
SA034162NAH20L1Control
SA034163NAH12L1Control
SA034164NAH18L1Control
SA034165NAH15L1Control
SA034166NAH5BL1Control
SA034167NAH4BL1Control
SA034168NAH11L1Control
SA034169NAH2BL1Control
SA034170NAH6BL1Control
SA034171NAH3BL1Control
SA034172NAH7BL1Control
SA034173NAH10BL1Control
SA034174NAH9BL1Control
SA034175NAH8BL1Control
SA034176GAH15L1Glaucomatous
SA034177GAH14L1Glaucomatous
SA034178GAH13L1Glaucomatous
SA034179GAH16L1Glaucomatous
SA034180GAH19L1Glaucomatous
SA034181GAH12L1Glaucomatous
SA034182GAH20L1Glaucomatous
SA034183GAH18L1Glaucomatous
SA034184GAH17L1Glaucomatous
SA034185GAH1BL1Glaucomatous
SA034186GAH5BL1Glaucomatous
SA034187GAH4BL1Glaucomatous
SA034188GAH3BL1Glaucomatous
SA034189GAH2BL1Glaucomatous
SA034190GAH6BL1Glaucomatous
SA034191GAH7BL1Glaucomatous
SA034192GAH10BL1Glaucomatous
SA034193GAH9BL1Glaucomatous
SA034194GAH8BL1Glaucomatous
SA034195GAH11L1Glaucomatous
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