Summary of Study ST000616
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000450. The data can be accessed directly via it's Project DOI: 10.21228/M8WC8S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000616 |
| Study Title | Human Aqueous Humor Phospholipids in Control and Glaucomatous eyes |
| Study Summary | An analysis of phospholipid (phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine and phosphatidylinositol) classes in human control and glaucomatous aqueous humor (AH). Lipid extraction was performed using a modification of the Bligh and Dyer method, protein concentrations were determined using the Bradford’s method, and select samples were confirmed with Densitometry of PHAST gels. Lipids were identified and subjected to ratiometric quantification using a TSQ Quantum Access Max triple quadrupole mass spectrometer utilizing precursor ion scan (PIS) or neutral ion loss scan (NLS) using appropriate class specific lipid standards in a two-step quantification process. Mass spectrometer data were analyzed using MzMine 2.23. |
| Institute | University of Miami |
| Last Name | Bhattacharya |
| First Name | Sanjoy |
| Address | 1638 NW 10th Avenue, Miami, Florida, 33136, USA |
| sbhattacharya@med.miami.edu | |
| Phone | 3054824103 |
| Submit Date | 2017-05-31 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | MS(Dir. Inf.) |
| Release Date | 2019-07-17 |
| Release Version | 1 |
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Project:
| Project ID: | PR000450 |
| Project DOI: | doi: 10.21228/M8WC8S |
| Project Title: | Phospholipid Profiling of Human Glaucomatous and Control Aqueous Humor and Trabecular Meshwork |
| Project Summary: | In this study we used a triple quadrupole mass spectrometer to investigate the phospholipids within human aqueous humor and trabecular meshwork. Our aim was to find potential differences in the lipid profiles between control and glaucomatous donors to better understand the disease. |
| Institute: | University of Miami |
| Last Name: | Bhattacharya |
| First Name: | Sanjoy |
| Address: | 1638 NW 10th Avenue, Miami, Florida, 33136, USA |
| Email: | sbhattacharya@med.miami.edu |
| Phone: | 3054824103 |
