Summary of Study ST000963

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000661. The data can be accessed directly via it's Project DOI: 10.21228/M87D53 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000963
Study TitleLipidomics of inflammation-induced optic nerve regeneration
Study Typeuntargeted LC-MS/MS profiling
Study SummaryIn adult mammals, retinal ganglion cells (RGCs) fail to regenerate their axons when damaged. As a result, RGCs die after acute injury and in progressive degenerative diseases such as glaucoma; such damage can lead to permanent vision loss and blindness. Little is known about the roles of lipids in axon injury and repair despite their fundamental importance in composition of cell membranes, myelin sheaths and mediation of signaling pathways. Study of the lipidome in the biology of optic nerve (ON) regeneration has been largely neglected. A better understanding of the roles that lipids play in RGC biology may enhance understanding of RGC-related diseases and point to novel treatments. Established experimental models of ON regeneration allow exploration of molecular determinants of RGC axon regenerative success and failure. In this study, we used high-resolution liquid chromatography-tandem mass spectrometry to analyze lipidomic profiles of the ON and retina in an ON crush model with and without intravitreal Zymosan injections to enhance regeneration. Our results reveal profound remodeling of retina and ON lipidomes that occur after injury. In the retina, Zymosan treatment largely abrogates widespread lipidome alterations. In the ON, Zymosan induces lipid profiles that are distinct from those observed in naïve and vehicle-injected crush controls. We have identified a number of lipid species, classes and fatty acids that may be involved in the mechanisms of axon damage and repair. Lipids upregulated during RGC regeneration may be interesting candidates for further functional studies.
Institute
University of Miami
DepartmentOphthalmology, Bascom Palmer Eye Institute
LaboratorySanjoy K. Bhattacharya Lab
Last NameBhattacharya
First NameSanjoy
Address900 NW 17th St, Miami, FL 33136, USA
Emailsbhattacharya@med.miami.edu
Phone3054824103
Submit Date2018-04-17
Num Groups9
Total Subjects28
Num Males28
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2018-09-27
Release Version1
Sanjoy Bhattacharya Sanjoy Bhattacharya
https://dx.doi.org/10.21228/M87D53
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA057645DS_30naïve control
SA057646DS_40naïve control
SA057647DS_12naïve control
SA057648DS_29naïve control
SA057649DS_19naïve control
SA057650DS_21naïve control
SA057651DS_20naïve control
SA057652DS_1naïve control
SA057653DS_28naïve control
SA057654DS_11naïve control
SA057655DS_2naïve control
SA057656DS_3naïve control
SA057657DS_38naïve control
SA057658DS_39naïve control
SA057659DS_10naïve control
SA057677DS_42optic nerve crush + vehicle
SA057678DS_43optic nerve crush + vehicle
SA057679DS_41optic nerve crush + vehicle
SA057680DS_24optic nerve crush + vehicle
SA057681DS_13optic nerve crush + vehicle
SA057682DS_6optic nerve crush + vehicle
SA057683DS_5optic nerve crush + vehicle
SA057684DS_4optic nerve crush + vehicle
SA057685DS_14optic nerve crush + vehicle
SA057686DS_15optic nerve crush + vehicle
SA057687DS_32optic nerve crush + vehicle
SA057688DS_31optic nerve crush + vehicle
SA057689DS_23optic nerve crush + vehicle
SA057690DS_22optic nerve crush + vehicle
SA057691DS_33optic nerve crush + vehicle
SA057660DS_25optic nerve crush + Zymosan + CPT-cAMP
SA057661DS_36optic nerve crush + Zymosan + CPT-cAMP
SA057662DS_27optic nerve crush + Zymosan + CPT-cAMP
SA057663DS_34optic nerve crush + Zymosan + CPT-cAMP
SA057664DS_35optic nerve crush + Zymosan + CPT-cAMP
SA057665DS_26optic nerve crush + Zymosan + CPT-cAMP
SA057666DS_18optic nerve crush + Zymosan + CPT-cAMP
SA057667DS_8optic nerve crush + Zymosan + CPT-cAMP
SA057668DS_9optic nerve crush + Zymosan + CPT-cAMP
SA057669DS_7optic nerve crush + Zymosan + CPT-cAMP
SA057670DS_46optic nerve crush + Zymosan + CPT-cAMP
SA057671DS_47optic nerve crush + Zymosan + CPT-cAMP
SA057672DS_45optic nerve crush + Zymosan + CPT-cAMP
SA057673DS_44optic nerve crush + Zymosan + CPT-cAMP
SA057674DS_37optic nerve crush + Zymosan + CPT-cAMP
SA057675DS_17optic nerve crush + Zymosan + CPT-cAMP
SA057676DS_16optic nerve crush + Zymosan + CPT-cAMP
Showing results 1 to 47 of 47
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