Summary of Study ST001659

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001064. The data can be accessed directly via it's Project DOI: 10.21228/M85H6W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001659
Study TitleIdentify putative volatile biomarkers of Coccidioides spp. grown in vitro
Study TypeUntargeted metabolomics
Study SummaryValley fever (coccidioidomycosis) is an endemic fungal pneumonia of the North and South American deserts. The causative agents of Valley fever are the dimorphic fungi Coccidioides immitis and C. posadasii, which grow as mycelia in the environment and spherules within the lungs of vulnerable hosts. The current diagnostics for Valley fever are severely lacking due to poor sensitivity and invasiveness, contributing to a 23-day median time-to-diagnosis, and therefore new diagnostic tools are needed. We are working toward the development of a breath-based diagnostic for coccidioidomycosis, and in this initial study we characterized the volatile metabolomes (or volatilomes) of in vitro cultures of Coccidioides. Using solid-phase microextraction and comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GC×GC–TOFMS), we characterized the VOCs produced by six strains of each species during mycelial or spherule growth. We detected a total of 353 VOCs that were at least two-fold more abundant in a Coccidioides culture versus medium controls and found the volatile metabolome of Coccidioides is more dependent on growth phase (spherule versus mycelia) than on the species. The volatile profiles of C. immitis and C. posadasii have strong similarities, indicating that a single suite of Valley fever breath biomarkers can be developed to detect both species.
Institute
Arizona State University
DepartmentSchool of Life Sciences
LaboratoryBean Laboratory
Last NameBean
First NameHeather
AddressPO Box 874501 Tempe, AZ 85287
EmailHeather.D.Bean@asu.edu
Phone4807273395
Submit Date2021-01-22
PublicationsLifecycle dominates the volatilome character of the dimorphic fungus Coccidioides spp Emily A. Higgins Keppler, Heather L. Mead, Bridget M. Barker, Heather D. Bean bioRxiv 2021.01.15.426916; doi: https://doi.org/10.1101/2021.01.15.426916
Raw Data AvailableYes
Raw Data File Type(s)smp
Analysis Type DetailGC-MS
Release Date2021-03-15
Release Version1
Heather Bean Heather Bean
https://dx.doi.org/10.21228/M85H6W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Fungi; Subject species: Coccidioides posadasii;Coccidioides immitis (Factor headings shown in green)

mb_sample_id local_sample_id Culture tempurature Culture conditions
SA151948RMSCC2010 Mycelia_230°C normoxia
SA151949RMSCC3506 Mycelia_230°C normoxia
SA151950RMSCC2010 Mycelia_330°C normoxia
SA151951RMSCC2010 Mycelia_430°C normoxia
SA151952RMSCC3506 Mycelia_330°C normoxia
SA151953RMSCC2009 Mycelia_330°C normoxia
SA151954RMSCC2006 Mycelia_330°C normoxia
SA151955RS Mycelia_130°C normoxia
SA151956RMSCC2009 Mycelia_230°C normoxia
SA151957B3221 Mycelia_230°C normoxia
SA151958RMSCC2009 Mycelia_430°C normoxia
SA151959RMSCC3506 Mycelia_130°C normoxia
SA151960RMSCC2395 Mycelia_330°C normoxia
SA151961RMSCC2395 Mycelia_430°C normoxia
SA151962RMSCC3505 Mycelia_330°C normoxia
SA151963RMSCC3505 Mycelia_230°C normoxia
SA151964RMSCC2395 Mycelia_130°C normoxia
SA151965RMSCC3505 Mycelia_430°C normoxia
SA151966RMSCC2343 Mycelia_130°C normoxia
SA151967RMSCC2343 Mycelia_330°C normoxia
SA151968RMSCC2343 Mycelia_430°C normoxia
SA151969RMSCC2006 Mycelia_230°C normoxia
SA151970RMSCC2006 Mycelia_430°C normoxia
SA151971Silveira Mycelia_230°C normoxia
SA151972Control Mycelia_230°C normoxia
SA151973RS Mycelia_330°C normoxia
SA151974Control Mycelia_430°C normoxia
SA151975Silveira Mycelia_330°C normoxia
SA151976B3222 Mycelia_430°C normoxia
SA151977B3221 Mycelia_330°C normoxia
SA151978B3221 Mycelia_430°C normoxia
SA151979B3222 Mycelia_230°C normoxia
SA151980B3222 Mycelia_330°C normoxia
SA151981Silveira Mycelia_130°C normoxia
SA151982Control Mycelia_330°C normoxia
SA151983GT-166 Mycelia_430°C normoxia
SA151984GT-166 Mycelia_230°C normoxia
SA151985GT-166 Mycelia_330°C normoxia
SA151986RS Mycelia_430°C normoxia
SA151987RS Spherule_139°C 10% CO2
SA151988RMSCC3506 Spherule_339°C 10% CO2
SA151989Silveira Spherule_239°C 10% CO2
SA151990RMSCC2395 Spherule_239°C 10% CO2
SA151991Silveira Spherule_339°C 10% CO2
SA151992RMSCC3505 Spherule_139°C 10% CO2
SA151993RMSCC3506 Spherule_239°C 10% CO2
SA151994RMSCC3506 Spherule_139°C 10% CO2
SA151995RS Spherule_239°C 10% CO2
SA151996RMSCC3505 Spherule_339°C 10% CO2
SA151997Silveira Spherule_139°C 10% CO2
SA151998RS Spherule_339°C 10% CO2
SA151999RMSCC3505 Spherule_239°C 10% CO2
SA152000RMSCC2010 Spherule_139°C 10% CO2
SA152001Control Spherule_239°C 10% CO2
SA152002Control Spherule_139°C 10% CO2
SA152003Control Spherule_339°C 10% CO2
SA152004GT-166 Spherule_139°C 10% CO2
SA152005GT-166 Spherule_239°C 10% CO2
SA152006B3222 Spherule_339°C 10% CO2
SA152007B3222 Spherule_239°C 10% CO2
SA152008B3221 Spherule_139°C 10% CO2
SA152009B3221 Spherule_239°C 10% CO2
SA152010B3221 Spherule_339°C 10% CO2
SA152011B3222 Spherule_139°C 10% CO2
SA152012GT-166 Spherule_339°C 10% CO2
SA152013RMSCC2006 Spherule_139°C 10% CO2
SA152014RMSCC2343 Spherule_139°C 10% CO2
SA152015RMSCC2010 Spherule_339°C 10% CO2
SA152016RMSCC2343 Spherule_239°C 10% CO2
SA152017RMSCC2343 Spherule_339°C 10% CO2
SA152018RMSCC2395 Spherule _139°C 10% CO2
SA152019RMSCC2010 Spherule_239°C 10% CO2
SA152020RMSCC2009 Spherule_339°C 10% CO2
SA152021RMSCC2006 Spherule_239°C 10% CO2
SA152022RMSCC2006 Spherule_339°C 10% CO2
SA152023RMSCC2009 Spherule_139°C 10% CO2
SA152024RMSCC2009 Spherule_239°C 10% CO2
SA152025RMSCC2395 Spherule_139°C 10% CO2
Showing results 1 to 78 of 78
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