Summary of Study ST002967

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001846. The data can be accessed directly via it's Project DOI: 10.21228/M83H81 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002967
Study TitleLipidomics study of FASN inhibition in HT-29 and HCT 116 spheroids
Study SummaryCancerous cells synthesize most of their lipids de novo to keep up with their rapid growth and proliferation. Fatty acid synthase (FAS) is a key enzyme in the lipogenesis pathway that is upregulated in many cancers and has gained popularity as a druggable target of interest for cancer treatment. The first FAS inhibitor discovered, cerulenin, initially showed promise for chemotherapeutic purposes until it was observed that it had adverse side effects in mice. TVB2640 (Denifanstat), is part of the newer generation of inhibitors. With multiple generations of FAS inhibitors being developed, it is vital to understand their distinct molecular downstream effects to elucidate potential interactions in the clinic. Here, we profile the lipidome of two different colorectal cancer (CRC) spheroids treated with a generation 1 inhibitor (cerulenin) or a generation 2 inhibitor (TVB-2640). We observe that the cerulenin causes drastic changes to the spheroid morphology as well as alterations to the lipid droplets found within CRC spheroids. TVB-2640 causes higher abundances of polyunsaturated fatty acids (PUFAs) whereas cerulenin causes decreased abundance of PUFAs. The increase in PUFAs in TVB-2640 exposed spheroids indicates it is causing cells to die via a ferroptotic mechanism rather than a conventional apoptotic or necrotic mechanism.
Institute
The Ohio State University
DepartmentChemistry and Biochemistry
LaboratoryAmanda Hummon Lab
Last NameFries
First NameBrian
Address460 W 12th Ave, Columbus, OH 43210
Emailfries.94@osu.edu
Phone9375221195
Submit Date2023-11-08
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2024-04-02
Release Version1
Brian Fries Brian Fries
https://dx.doi.org/10.21228/M83H81
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA32296825HCT116_CER4Cerulenin
SA32296932HCT116_CER5Cerulenin
SA32297007HT29_CER1Cerulenin
SA32297111HCT116_CER2Cerulenin
SA32297214HT29_CER2Cerulenin
SA32297318HCT116_CER3Cerulenin
SA32297435HT29_CER5Cerulenin
SA32297521HT29_CER3Cerulenin
SA32297628HT29_CER4Cerulenin
SA32297704HCT116_CER1Cerulenin
SA32297822HT29_CTRL3Control
SA32297929HT29_CTRL4Control
SA32298005HCT116_CTRL1Control
SA32298115HT29_CTRL2Control
SA32298236HT29_CTRL5Control
SA32298319HCT116_CTRL3Control
SA32298408HT29_CTRL1Control
SA32298526HCT116_CTRL4Control
SA32298633HCT116_CTRL5Control
SA32298712HCT116_CTRL2Control
SA32298801POOLSamplePool1
SA32298902POOLSamplePool2
SA32299003POOLSamplePool3
SA32299110POOLSamplePool4
SA32299217POOLSamplePool5
SA32299324POOLSamplePool6
SA32299431POOLSamplePool7
SA32299538POOLSamplePool8
SA32299623HT29_TVB3TVB-2640
SA32299720HCT116_TVB3TVB-2640
SA32299827HCT116_TVB4TVB-2640
SA32299934HCT116_TVB5TVB-2640
SA32300013HCT116_TVB2TVB-2640
SA32300106HCT116_TVB1TVB-2640
SA32300230HT29_TVB4TVB-2640
SA32300316HT29_TVB2TVB-2640
SA32300409HT29_TVB1TVB-2640
SA32300537HT29_TVB5TVB-2640
Showing results 1 to 38 of 38
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