Summary of study ST000257

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000209. The data can be accessed directly via it's Project DOI: 10.21228/M8CP4Q This work is supported by NIH grant, U2C- DK119886.

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Study IDST000257
Study TitleNIH WCMC Pilot & Feasibility Project: “Metabolite changes associated with weight loss”
Study TypeFeeding study
Study SummaryTargeted metabolomic analyses of oxylipins, endocannabinoids, and ceramides were performed on 18 mouse liver, adipose, hypothalamus, plasma, and muscle samples collected from mice euthanized at 18 weeks following consumption of three diet regimens that induced lean, obese, and weight loss phenotypes. Samples were analyzed by UPLC-MS/MS using a Waters Acquity UPLC and detected on an API 4000 QTrap (AB Sciex, Framingham, MA, USA) by multiple reaction monitoring (MRM) after negative mode electrospray ionization.
Institute
University of California, Davis
DepartmentNutrition
LaboratoryNewman Lab
Last NameNewman
First NameJohn
Address430 W. Health Sciences Dr., Davis, CA 95616
Emailjohn.newman@ars.usda.gov
Phone+1-530-752-1009
Submit Date2015-01-16
Num Groups3
Total Subjects18
Raw Data AvailableYes
Raw Data File Type(s).mzML
Uploaded File Size128 MB
Analysis Type DetailLC-MS
Release Date2015-09-24
Release Version1
John Newman John Newman
https://dx.doi.org/10.21228/M8CP4Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000406 AN000407 AN000408
Analysis type MS MS MS
Chromatography type
Chromatography system Waters Acquity Waters Acquity Waters Acquity
Column
MS Type ESI ESI ESI
MS instrument type Triple quadrupole Triple quadrupole Triple quadrupole
MS instrument name ABI Sciex API 4000 QTrap ABI Sciex API 4000 QTrap ABI Sciex API 4000 QTrap
Ion Mode NEGATIVE POSITIVE POSITIVE
Units % Area Ratio nM nM

MS:

MS ID:MS000348
Analysis ID:AN000406
Instrument Name:ABI Sciex API 4000 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:See protocol file
Ion Mode:NEGATIVE
  
MS ID:MS000349
Analysis ID:AN000407
Instrument Name:ABI Sciex API 4000 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:See protocol file
Ion Mode:POSITIVE
  
MS ID:MS000350
Analysis ID:AN000408
Instrument Name:ABI Sciex API 4000 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:See protocol file
Ion Mode:POSITIVE
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