Summary of Study ST001653

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001059. The data can be accessed directly via it's Project DOI: 10.21228/M8T69T This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001653
Study TitleMucosal-Associated-Invariant-T (MAIT) cells stimulated with IL-12/IL-12, anti-CD3/CD28 or both for 16 hours
Study SummaryThe untargeted metabolomics analysis was performed after metabolite extraction from vital cells. The main object of the study was to define the activation of MAIT cells on the molecular level.
Institute
Helmholtz Centre for Environmental Research
Last NameEngelmann
First NameBeatrice
AddressPermoserstraße 15, Leipzig, Saxony, 03418, Germany
Emailbeatrice.engelmann@ufz.de
Phone00493412351099
Submit Date2021-01-12
Num Groups4
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2021-02-01
Release Version1
Beatrice Engelmann Beatrice Engelmann
https://dx.doi.org/10.21228/M8T69T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002700
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290
Column Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6540 QTOF
Ion Mode POSITIVE
Units peak area

MS:

MS ID:MS002498
Analysis ID:AN002700
Instrument Name:Agilent 6540 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Mass Hunter software was used to obtain raw data.Eluted metabolites were measured with the QTOF operated in centroid mode. Full scan data was generated with a scan range of 60-1600 m/z in positive ionization mode. Out of the survey scan the 5 most abundant precursor ions with charge state = 1 were subjected to fragmentation. The dynamic exclusion time was set at 30 s. Peak picking and database search was done using Progenesis QI software.
Ion Mode:POSITIVE
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