Summary of Study ST001653

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001059. The data can be accessed directly via it's Project DOI: 10.21228/M8T69T This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST001653
Study Title	Mucosal-Associated-Invariant-T (MAIT) cells stimulated with IL-12/IL-12, anti-CD3/CD28 or both for 16 hours
Study Summary	The untargeted metabolomics analysis was performed after metabolite extraction from vital cells. The main object of the study was to define the activation of MAIT cells on the molecular level.
Institute	Helmholtz Centre for Environmental Research
Last Name	Engelmann
First Name	Beatrice
Address	Permoserstraße 15, Leipzig, Saxony, 03418, Germany
Email	beatrice.engelmann@ufz.de
Phone	00493412351099
Submit Date	2021-01-12
Num Groups	4
Raw Data Available	Yes
Raw Data File Type(s)	d
Analysis Type Detail	LC-MS
Release Date	2021-02-01
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001736
Sampleprep Summary:	Cells were quenched 3 times with 1 ml ice-cold 0.9% sodium chloride. After removing the quenching solution, cells were resuspended in 100 µl ice-cold acetonitrile followed by 100 µl ice-cold Milli-Q water. After vortexing for 1 min, cells were centrifuged (14000 rpm, 4 °C, 10 min). Supernatants were transferred to new tubes. Intracellular metabolites were extracted again with 500 µl Methanol:ACN:Milli-Q water (2:3:1). After centrifugation (14000 rpm, 4 ° C, 10 min) both supernatants were combined, evaporated to dryness and stored at -80 °C until measurement.

Sampleprep ID:

SP001736

Sampleprep Summary:

Cells were quenched 3 times with 1 ml ice-cold 0.9% sodium chloride. After removing the quenching solution, cells were resuspended in 100 µl ice-cold acetonitrile followed by 100 µl ice-cold Milli-Q water. After vortexing for 1 min, cells were centrifuged (14000 rpm, 4 °C, 10 min). Supernatants were transferred to new tubes. Intracellular metabolites were extracted again with 500 µl Methanol:ACN:Milli-Q water (2:3:1). After centrifugation (14000 rpm, 4 ° C, 10 min) both supernatants were combined, evaporated to dryness and stored at -80 °C until measurement.