Summary of Study ST002060
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001304. The data can be accessed directly via it's Project DOI: 10.21228/M8570V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002060 |
| Study Title | Pollen metabolomics using Arabidopsis thaliana: Comparison of pollen at mature, hydration and germination stage |
| Study Type | Untargeted Metabolomics |
| Study Summary | In this study, we investigated the differential metabolic pathway enrichment among mature, hydrated, and germinated pollen using untargeted metabolomics analysis. Integration of publicly available transcriptome with presented metabolome data revealed starch and sucrose metabolism was significantly increased during pollen hydration and germination. The alterations in central metabolism focusing on sugar, fatty acids, and lipids were analyzed in detail. Several metabolites, including palmitic acid, oleic acid, linolenic acid, quercetin, luteolin/kaempferol, and γ-aminobutyric acid (GABA), were elevated in the hydrated pollen, suggesting a potential role in activating pollen tube emergence. The metabolite levels of mature, hydrated, and germinated pollen, presented in this work provide insights on the molecular basis of pollen germination. |
| Institute | University of Illinois Urbana-Champaign |
| Department | Department of Plant Biology |
| Laboratory | Li-Qing Chen Lab |
| Last Name | Kambhampati |
| First Name | Shrikaar |
| Address | 975 North Warson Road, St. Louis, MO 63132 |
| shrikaar.k@gmail.com | |
| Phone | 3144025550 |
| Submit Date | 2022-01-17 |
| Num Groups | 3 |
| Total Subjects | 12 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-02-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003354 | AN003355 | AN003356 | AN003357 | AN003358 | AN003359 |
|---|---|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Eksigent Ekspert microLC 200 | Eksigent Ekspert microLC 200 | Eksigent Ekspert microLC 200 | Eksigent Ekspert microLC 200 | Eksigent Ekspert microLC 200 | Eksigent Ekspert microLC 200 |
| Column | SeQuant Zic-HILIC (150 x 0.5mm,5um) | SeQuant Zic-HILIC (150 x 0.5mm,5um) | Higgins Analytica Targa C18 (150 x 0.3mm,3.5um) | Higgins Analytica Targa C18 (150 x 0.3mm,3.5um) | Machery-Nagel Nucleodur C8 (150 x 0.5mm,5um) | Machery-Nagel Nucleodur C8 (150 x 0.5mm,5um) |
| MS Type | ESI | ESI | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Intensity | Intensity | Intensity | Intensity | Intensity | Intensity |
MS:
| MS ID: | MS003123 |
| Analysis ID: | AN003354 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | All parameters listed in the attached document |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | MS_DataAcquisition.docx |
| MS ID: | MS003124 |
| Analysis ID: | AN003355 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | All parameters listed in the attached document |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | MS_DataAcquisition.docx |
| MS ID: | MS003125 |
| Analysis ID: | AN003356 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | All parameters listed in the attached document |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | MS_DataAcquisition.docx |
| MS ID: | MS003126 |
| Analysis ID: | AN003357 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | All parameters listed in the attached document |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | MS_DataAcquisition.docx |
| MS ID: | MS003127 |
| Analysis ID: | AN003358 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | All parameters listed in the attached document |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | MS_DataAcquisition.docx |
| MS ID: | MS003128 |
| Analysis ID: | AN003359 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | All parameters listed in the attached document |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | MS_DataAcquisition.docx |
