Summary of Study ST002060

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001304. The data can be accessed directly via it's Project DOI: 10.21228/M8570V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002060
Study TitlePollen metabolomics using Arabidopsis thaliana: Comparison of pollen at mature, hydration and germination stage
Study TypeUntargeted Metabolomics
Study SummaryIn this study, we investigated the differential metabolic pathway enrichment among mature, hydrated, and germinated pollen using untargeted metabolomics analysis. Integration of publicly available transcriptome with presented metabolome data revealed starch and sucrose metabolism was significantly increased during pollen hydration and germination. The alterations in central metabolism focusing on sugar, fatty acids, and lipids were analyzed in detail. Several metabolites, including palmitic acid, oleic acid, linolenic acid, quercetin, luteolin/kaempferol, and γ-aminobutyric acid (GABA), were elevated in the hydrated pollen, suggesting a potential role in activating pollen tube emergence. The metabolite levels of mature, hydrated, and germinated pollen, presented in this work provide insights on the molecular basis of pollen germination.
Institute
University of Illinois Urbana-Champaign
DepartmentDepartment of Plant Biology
LaboratoryLi-Qing Chen Lab
Last NameKambhampati
First NameShrikaar
Address975 North Warson Road, St. Louis, MO 63132
Emailshrikaar.k@gmail.com
Phone3144025550
Submit Date2022-01-17
Num Groups3
Total Subjects12
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2022-02-07
Release Version1
Shrikaar Kambhampati Shrikaar Kambhampati
https://dx.doi.org/10.21228/M8570V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Subject:

Subject ID:SU002142
Subject Type:Plant
Subject Species:Arabidopsis thaliana
Taxonomy ID:3702
Genotype Strain:Col-0
Age Or Age Range:Mature pollen, Pollen hydration Stage (45 min), Pollen germination stage (4 hours)
Species Group:Pollen
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