Summary of Study ST002177
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001385. The data can be accessed directly via it's Project DOI: 10.21228/M8PT3H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002177 |
| Study Title | Multiomics Analyses Reveal the Central Role of Pentose Phosphate Pathway in Resident Thymic Macrophages to Cope with Efferocytosis-Associated Stress |
| Study Summary | Tissue-resident macrophages (TRMs) are heterogeneous cell populations found throughout the body. Depending on their location, they perform diverse functions maintaining tissue homeostasis and providing immune surveillance. To survive and function within, TRMs adapt metabolically to the distinct microenvironments. However, little is known about the metabolic signatures of TRMs. The thymus provides a nurturing milieu for developing thymocytes yet efficiently removes those that failed the selection, relying on the TRMs – resident thymic macrophages (TMφs). This study harnesses multiomics analyses to characterize TMφs and unveils their unique metabolic features. We find that the pentose phosphate pathway (PPP) is preferentially activated in TMφs, responding to the reduction-oxidation demands associated with the efferocytosis of dying thymocytes. The blockade of PPP in Mφs leads to decreased efferocytosis, which can be rescued by ROS scavengers. Our study reveals the key role of PPP in TMφs and underscores the importance of metabolic adaptation in supporting Mφ efferocytosis. |
| Institute | National Yang Ming Chiao Tung University |
| Department | Institute of Microbiology and Immunology |
| Laboratory | Chai-Lin Hsu |
| Last Name | Hsu |
| First Name | Chia-Lin |
| Address | R309, Biomedical Building, NYCU, No. 155, Sec. 2, Linong St., Beitou Dist. Taipei 112, Taiwan |
| clhsu@nycu.edu.tw | |
| Phone | +886-2-2826-7000 ext:65619 |
| Submit Date | 2022-05-21 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-06-09 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003565 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Xevo-G2-XS |
| Ion Mode | NEGATIVE |
| Units | peak area |
MS:
| MS ID: | MS003322 |
| Analysis ID: | AN003565 |
| Instrument Name: | Waters Xevo-G2-XS |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Raw data is processed by Progenesis QI software. The features were matched to the KEGG compounds through Chemspider while the mass error was limited to 15 ppm. |
| Ion Mode: | NEGATIVE |
