Summary of Study ST002752
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001509. The data can be accessed directly via it's Project DOI: 10.21228/M8N71K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002752 |
| Study Title | Biomolecular condensates create phospholipid-enriched microenvironments (Part 7 - reversed phase experiment set 2) |
| Study Type | Metabolomes of in vitro synthesized condensates |
| Study Summary | Proteins and RNA are able to phase separate from the aqueous cellular environment to form sub-cellular compartments called condensates. This process results in a protein-RNA mixture that is chemically distinct from the surrounding aqueous phase. In this project we used mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and cellular metabolites and identified metabolites enriched in the condensate phase. These proteins included SARS-CoV-2 nucleocapsid, as well as low complexity domains of MED1 and HNRNPA1. In this sub-study, we examined the metabolomes of the mouse liver samples that were used to conduct the condensate metabolome experiment described above. |
| Institute | Cornell University |
| Department | Department of Pharmacology |
| Laboratory | Dr. Samie Jaffrey |
| Last Name | Dumelie |
| First Name | Jason |
| Address | 1300 York Ave, LC-524, New York City, NY |
| jdumes98@gmail.com | |
| Phone | 6465690174 |
| Submit Date | 2023-06-22 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | xml |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-07-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004465 | AN004466 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent Model 1290 Infinity II liquid chromatography system | Agilent Model 1290 Infinity II liquid chromatography system |
| Column | Cogent Diamond Hydride (150 × 2.1 mm, 4um) | Cogent Diamond Hydride (150 × 2.1 mm, 4um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Bruker Impact HD | Bruker Impact HD |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Ion abundance (max peak height) | Ion abundance (max peak height) |
MS:
| MS ID: | MS004212 |
| Analysis ID: | AN004465 |
| Instrument Name: | Bruker Impact HD |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The Bruker Impact II QTOF was equipped with a vacuum insulated probe heated electrospray ionization source (VIP-HESI) (Bruker Daltonics, Billerica, USA) to identify representative lipid structures using auto-MS/MS with and without scheduled precursor list fragmentation. Fragments were compared with those deposited in LIPID MAPS, HMDB and MassBank |
| Ion Mode: | POSITIVE |
| MS ID: | MS004213 |
| Analysis ID: | AN004466 |
| Instrument Name: | Bruker Impact HD |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The Bruker Impact II QTOF was equipped with a vacuum insulated probe heated electrospray ionization source (VIP-HESI) (Bruker Daltonics, Billerica, USA) to identify representative lipid structures using auto-MS/MS with and without scheduled precursor list fragmentation. Fragments were compared with those deposited in LIPID MAPS, HMDB and MassBank. |
| Ion Mode: | NEGATIVE |
