Summary of Study ST002752
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001509. The data can be accessed directly via it's Project DOI: 10.21228/M8N71K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002752 |
Study Title | Biomolecular condensates create phospholipid-enriched microenvironments (Part 7 - reversed phase experiment set 2) |
Study Type | Metabolomes of in vitro synthesized condensates |
Study Summary | Proteins and RNA are able to phase separate from the aqueous cellular environment to form sub-cellular compartments called condensates. This process results in a protein-RNA mixture that is chemically distinct from the surrounding aqueous phase. In this project we used mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and cellular metabolites and identified metabolites enriched in the condensate phase. These proteins included SARS-CoV-2 nucleocapsid, as well as low complexity domains of MED1 and HNRNPA1. In this sub-study, we examined the metabolomes of the mouse liver samples that were used to conduct the condensate metabolome experiment described above. |
Institute | Cornell University |
Department | Department of Pharmacology |
Laboratory | Dr. Samie Jaffrey |
Last Name | Dumelie |
First Name | Jason |
Address | 1300 York Ave, LC-524, New York City, NY |
jdumes98@gmail.com | |
Phone | 6465690174 |
Submit Date | 2023-06-22 |
Raw Data Available | Yes |
Raw Data File Type(s) | xml |
Analysis Type Detail | LC-MS |
Release Date | 2023-07-10 |
Release Version | 1 |
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Project:
Project ID: | PR001509 |
Project DOI: | doi: 10.21228/M8N71K |
Project Title: | Biomolecular condensates create phospholipid-enriched microenvironments |
Project Type: | Metabolomics of in vitro condensates |
Project Summary: | Proteins and RNA are able to phase separate from the aqueous cellular environment to form sub-cellular compartments called condensates. This process results in a protein-RNA mixture that is chemically distinct from the surrounding aqueous phase. Here we use mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and cellular metabolites and identified metabolites enriched in the condensate phase. These proteins included SARS-CoV-2 nucleocapsid, as well as low complexity domains of MED1 and HNRNPA1. |
Institute: | Cornell University |
Department: | Department of Pharmacology |
Laboratory: | Dr. Samie Jaffrey |
Last Name: | Dumelie |
First Name: | Jason |
Address: | 1300 York Ave, LC-524, New York City, NY |
Email: | jdumes98@gmail.com |
Phone: | 6465690174 |
Funding Source: | This work was supported by the National Institutes of Health grants R35NS111631 and R01CA186702 (S.R.J.); R01AR076029, R21ES032347 and R21NS118633 (Q.C.); and NIH P01 HD067244 and support from the Starr Cancer Consortium I13-0037 (S.S.G.). |
Publications: | Under revision |
Contributors: | Jason G. Dumelie, Qiuying Chen, Dawson Miller, Nabeel Attarwala, Steven S. Gross and Samie R. Jaffrey1 |