Summary of Study ST000102
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000094. The data can be accessed directly via it's Project DOI: 10.21228/M8X309 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000102 |
| Study Title | Cold adaptation shapes the robustness of metabolic networks in Drosophila melanogaster |
| Study Type | Time course during cold exposure for four genetic lines of flies |
| Study Summary | Metabolites of cold hardy versus cold susceptible flies were compared using N less than R-based metabolomics. We used 8 replicates per line (2 hardy lines, two susceptible lines), and sampled each line at three time points (before, during and after cold), giving rise to 96 samples total. |
| Institute | University of Florida |
| Department | Entomology and Nematology |
| Last Name | Williams |
| First Name | Caroline |
| cmw@berkeley.edu | |
| Submit Date | 2014-09-26 |
| Num Groups | 4 |
| Total Subjects | 96 |
| Raw Data Available | Yes |
| Uploaded File Size | 151 M |
| Analysis Type Detail | NMR |
| Release Date | 2015-02-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
NMR:
| NMR ID: | NM000036 |
| Analysis ID: | AN000169 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Field Frequency Lock: | -6958.1 |
| Standard Concentration: | 1 mM TMSP |
| Spectrometer Frequency: | 700 MHz |
| NMR Probe: | Room Temperature 5-mm triple resonance probe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5 mm |
| Shimming Method: | topshim (automatic shimming) |
| Pulse Sequence: | noesypr1d |
| Water Suppression: | presaturation |
| Pulse Width: | 9.4-9.6 ?s |
| Power Level: | -1.00 dB |
| Receiver Gain: | 200 |
| Offset Frequency: | 3294.98 Hz |
| Presaturation Power Level: | 53.64 dB |
| Chemical Shift Ref Cpd: | TMSP |
| Temperature: | 298 K |
| Number Of Scans: | 120 |
| Dummy Scans: | 4 |
| Acquisition Time: | 2.9 s |
| Relaxation Delay: | 4 s |
| Spectral Width: | 16.0872 ppm |
| Num Data Points Acquired: | 65536 |
| Real Data Points: | 32768 |
| Line Broadening: | 0.3 Hz |
| Zero Filling: | 2x |
| Apodization: | exponential multiply (em) |
| Baseline Correction Method: | abs (automatic baseline correction with integration (1D)) |
| Chemical Shift Ref Std: | 0.00 ppm |
| NMR ID: | NM000037 |
| Analysis ID: | AN000170 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 2D 1H-13C HSQC |
| Field Frequency Lock: | -6958.1 |
| Standard Concentration: | 1 mM TMSP |
| Spectrometer Frequency: | 700 MHz |
| NMR Probe: | Room Temperature 5-mm triple resonance probe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5 mm |
| Shimming Method: | topshim (automatic shimming) |
| Pulse Sequence: | HSQCEDETGPSISP2.2 |
| Pulse Width: | 9.4-9.6 ?s |
| Power Level: | f1 -1.00 dB, f2 -4.13 dB |
| Receiver Gain: | 2050 |
| Offset Frequency: | f2 3294.95 Hz, f1 14081.41 Hz |
| Chemical Shift Ref Cpd: | TMSP |
| Temperature: | 298 K |
| Number Of Scans: | 256 |
| Dummy Scans: | 256 |
| Acquisition Time: | f2 0.13317 s, f1 0.0083333 s |
| Relaxation Delay: | 1.5 s |
| Spectral Width: | f2 10.9888, f1 180.00 |
| Num Data Points Acquired: | f2 2048, f1 528 |
| Real Data Points: | f2 4096, f1 2048 |
| Line Broadening: | f2 1.0 Hz, f1 0.3 Hz |
| Zero Filling: | f2 1x, f1 4x |
| Apodization: | f2 QSINE, f1 QSINE |
| Chemical Shift Ref Std: | 0.00, 0.00 ppm |
