Summary of Study ST000102
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000094. The data can be accessed directly via it's Project DOI: 10.21228/M8X309 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000102 |
| Study Title | Cold adaptation shapes the robustness of metabolic networks in Drosophila melanogaster |
| Study Type | Time course during cold exposure for four genetic lines of flies |
| Study Summary | Metabolites of cold hardy versus cold susceptible flies were compared using N less than R-based metabolomics. We used 8 replicates per line (2 hardy lines, two susceptible lines), and sampled each line at three time points (before, during and after cold), giving rise to 96 samples total. |
| Institute | University of Florida |
| Department | Entomology and Nematology |
| Last Name | Williams |
| First Name | Caroline |
| cmw@berkeley.edu | |
| Submit Date | 2014-09-26 |
| Num Groups | 4 |
| Total Subjects | 96 |
| Raw Data Available | Yes |
| Uploaded File Size | 151 M |
| Analysis Type Detail | NMR |
| Release Date | 2015-02-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000117 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | sample_prep_protocol_24Sept2014.docx sample_spiking_protocol_24Sept2014.docx |
| Sampleprep Protocol Comments: | Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37: 911-7. |
| Processing Method: | lyophilization, homogenization, solvent extraction |
| Processing Storage Conditions: | store at -20C |
| Extraction Method: | modified Bligh and Dyer protocol |
| Extract Concentration Dilution: | 2:2:1.8 (methanol:chloroform:water) |
| Extract Storage: | -20C |
| Sample Resuspension: | Samples were re-suspended in 620 ?l buffer (100 mM potassium phosphate [pH 7.3], 0.1% sodium azide, 1 mM trimethylsilylproprionate [TMSP] in D2O) |
| Sample Spiking: | 6 internal standards (proline, ?-alanine, tyrosine, histidine, phosphocholine, and 3-hydroxykynurenine) added: 6 ?L of standards were added to a concentration of 2x the Chenomx-determined concentration for each metabolite |
