Summary of Study ST001375
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000941. The data can be accessed directly via it's Project DOI: 10.21228/M8296W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001375 |
Study Title | Fructosamine-3-kinase (FN3K) KO in HepG2 liver cancer cells |
Study Summary | Fructosamine-3-kinases (FN3Ks) are a family of metabolic kinases which are evolutionarily related to eukaryotic protein kinases. Aberrant regulation of these kinases by altered redox homeostasis is a major contributing factor in aging and disease. However, the mechanisms of regulation and cellular functions of these kinases are not known. Bioinformatic analyses of cancer cell lines identified significant overexpression of FN3K in liver and eye cancer cells. To assess the functional significance of this increased expression, a CRISPR knockout of FN3K (FN3K-KO) was generated in the HepG2 liver cancer cell line. The metabolome was compared between FN3K-KO and WT HepG2 cells using untargeted 1H NMR metabolomics. This revealed significant differences in several metabolites that suggest a role for FN3K in regulating redox and energy balance in HepG2 cells. |
Institute | University of Georgia |
Department | Complex Carbohydrate Research Center |
Laboratory | Edison |
Last Name | Colonna |
First Name | Maxwell |
Address | 315 Riverbend Rd, Athens, GA 30602 |
maxwellbaca@uga.edu | |
Phone | 7065420257 |
Submit Date | 2020-05-08 |
Num Groups | 2 |
Publications | A redox-active switch in Fructosamine-3-kinases expands the regulatory repertoire of the protein kinase super-family |
Raw Data Available | Yes |
Raw Data File Type(s) | fid |
Analysis Type Detail | NMR |
Release Date | 2020-07-07 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
NMR:
NMR ID: | NM000164 |
Analysis ID: | AN002295 |
Instrument Name: | Bruker Avance |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Field Frequency Lock: | Deuterium |
Spectrometer Frequency: | 800 MHz |
NMR Probe: | 3mm Cryoprobe |
NMR Solvent: | D2O Phosphate buffer |
NMR Tube Size: | 3mm SampleJet |
Shimming Method: | Topshim |
Pulse Sequence: | noesypr1d |
Water Suppression: | Presaturation |
Pulse Width: | 8.22 us |
Power Level: | 11.117 W |
Offset Frequency: | 3762.64 |
Presaturation Power Level: | 3.00E-05 |
Chemical Shift Ref Cpd: | DSS |
Temperature: | 27 |
Number Of Scans: | 128 |
Dummy Scans: | 4 |
Acquisition Time: | 1.24 s |
Relaxation Delay: | 2 s |
Spectral Width: | 16.4533 ppm |
Num Data Points Acquired: | 32768 |
Line Broadening: | 0.3 Hz |
Baseline Correction Method: | Polynomial order 5 |