Summary of Study ST001375

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000941. The data can be accessed directly via it's Project DOI: 10.21228/M8296W This work is supported by NIH grant, U2C- DK119886.


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Study IDST001375
Study TitleFructosamine-3-kinase (FN3K) KO in HepG2 liver cancer cells
Study SummaryFructosamine-3-kinases (FN3Ks) are a family of metabolic kinases which are evolutionarily related to eukaryotic protein kinases. Aberrant regulation of these kinases by altered redox homeostasis is a major contributing factor in aging and disease. However, the mechanisms of regulation and cellular functions of these kinases are not known. Bioinformatic analyses of cancer cell lines identified significant overexpression of FN3K in liver and eye cancer cells. To assess the functional significance of this increased expression, a CRISPR knockout of FN3K (FN3K-KO) was generated in the HepG2 liver cancer cell line. The metabolome was compared between FN3K-KO and WT HepG2 cells using untargeted 1H NMR metabolomics. This revealed significant differences in several metabolites that suggest a role for FN3K in regulating redox and energy balance in HepG2 cells.
University of Georgia
DepartmentComplex Carbohydrate Research Center
Last NameColonna
First NameMaxwell
Address315 Riverbend Rd, Athens, GA 30602
Submit Date2020-05-08
Num Groups2
PublicationsA redox-active switch in Fructosamine-3-kinases expands the regulatory repertoire of the protein kinase super-family
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2020-07-07
Release Version1
Maxwell Colonna Maxwell Colonna application/zip

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Sample Preparation:

Sampleprep ID:SP001457
Sampleprep Summary:Aqueous metabolites were extracted by vortexing cell pellets in the extraction solvent, pelleting cell debris and collecting the supernatant. Approximately 10% of the supernatant volume was taken from each sample to form an internal pooled sample. The solvent was then evaporated to produce dried extracts.
Sampleprep Protocol Filename:cell_protocol.pdf
Processing Storage Conditions:On ice
Extraction Method:Vortex
Extract Enrichment:Polar
Extract Storage:-80℃
Sample Resuspension:Deuterium oxide phosphate buffer