Summary of Study ST001871
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001181. The data can be accessed directly via it's Project DOI: 10.21228/M82418 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001871 |
| Study Title | Untargeted LC-MS metabolomics analysis of cecal content of mice treated with TCDD vs. vehicle control (part I) |
| Study Type | Untargeted metabolomics |
| Study Summary | Six-week-old female wildtype (WT) C57BL/6 mice were administered a single 100µl intraperitoneal injection containing sterile corn oil (VEH group) or an intraperitoneal injection of 10µg/kg TCDD suspended within sterile corn oil (TCDD group). At the 72h time point following TCDD or VEH exposure, the mice were humanely euthanized by an overdose of inhaled isoflurane. During necropsy, cecal content and blood serum samples were collected for untargeted metabolomics profiling. |
| Institute | University of South Carolina School of Medicine |
| Department | Department of Pathology |
| Laboratory | (On behalf of) Mitzi Nagarkatti Lab |
| Last Name | Lai |
| First Name | Yunjia |
| Address | 135 Dauer Drive, Chapel Hill, NC 27599 |
| yunjia.lai@outlook.com | |
| Phone | 919-480-5489 |
| Submit Date | 2021-07-16 |
| Num Groups | 2 |
| Total Subjects | 20 |
| Num Females | 20 |
| Publications | Chemosphere |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-08-30 |
| Release Version | 1 |
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Project:
| Project ID: | PR001181 |
| Project DOI: | doi: 10.21228/M82418 |
| Project Title: | Effects of Acute 2,3,7,8-Tetrachlorodibenzo-p-dioxin Exposure on the Circulating and Cecal Metabolome Profile |
| Project Type: | Untargeted metabolomics |
| Project Summary: | 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a polyhalogenated planar hydrocarbon belonging to a group of highly toxic and persistent environmental contaminants known as “dioxins”. TCDD is an animal teratogen and carcinogen that is well characterized for causing immunosuppression through activation of Aryl Hydrocarbon Receptor (AHR). In the current study, we investigated the effect of exposure of mice to an acute dose of TCDD on the metabolic profile within the serum and cecal contents to better define the effects of TCDD on host physiology. Our findings demonstrated that within the circulating metabolome following acute TCDD, there was significant dysregulation in the metabolism of bioactive lipids, amino acids, and carbohydrates when compared to the VEH treated mice. These wide-spread changes in metabolite abundance were identified to regulate host immunity via modulating Nuclear Factor-Kappa B (NF-κB) and Extracellular Signal‑Regulated Protein Kinase (ERK1/2) activity, and work as biomarkers for a variety of organ injuries and dysfunctions that follow TCDD exposure. Within the cecal content, of mice exposed to TCDD, we were able to detect changes in inflammatory markers that regulate NF-κB, markers of injury-related inflammation, and changes in lysine degradation, nicotinamide metabolism, and butanoate metabolism, which suggested an immediate suppression microbial metabolism. Collectively, these results demonstrate that acute TCDD exposure results in immediate irregularities in the circulating and intestinal metabolome which likely contributes to TCDD toxicity and can be used as biomarkers for the early detection of individual exposure. |
| Institute: | University of South Carolina School of Medicine |
| Department: | Department of Pathology |
| Laboratory: | On behalf of Dr. Mitzi Nagarkatti Lab |
| Last Name: | Lai |
| First Name: | Yunjia |
| Address: | 1104 MHRC, 135 Dauer Dr., Chapel Hill, NC, 27599, USA |
| Email: | yunjia.lai@outlook.com |
| Phone: | 9194805489 |
| Funding Source: | National Institute of Health: P01AT003961, P20GM103641, R01ES030144, R01AI129788 and R01AI123947 |
| Project Comments: | This is supporting metabolomics data for the publication in Chemosphere. |
| Publications: | Chemosphere |
