Summary of Study ST000056
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000053. The data can be accessed directly via it's Project DOI: 10.21228/M8C883 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000056 |
| Study Title | Environmental impact on metabolomics and food allergy |
| Study Type | Metabolomics |
| Study Summary | This metabolomics pilot and feasibility (P&F) was conducted to determine if varying the levels of environmental LPS exposure and immunization with the vaccine adjuvant alum alters host metabolism. Additionally, the metabolomics will identify biomarkers that can predict allergic disease development and or disease severity after a peanut challenge in hypersensitive mice. Information gained from the proposed studies may allow for the identification of individuals who are at risk or not at risk for the development of allergic disease. Furthermore, completion of these studies may lead to identification of biological targets that may be used to develop novel therapies to treat or prevent allergic disease via future funding. |
| Institute | University of North Carolina |
| Department | Systems and Translational Sciences |
| Laboratory | Sumner Lab |
| Last Name | Sumner |
| First Name | Susan |
| Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
| susan_sumner @unc.edu | |
| Phone | 704-250-5066 |
| Submit Date | 2014-05-31 |
| Num Groups | 2 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Uploaded File Size | 68 M |
| Analysis Type Detail | NMR |
| Release Date | 2015-06-01 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP000071 |
| Sampleprep Summary: | Frozen fecal samples were transferred into numbered homogenizer bead tubes (ceramic) and diluted with D2O (Sigma-Aldrich, St. Louis, MO). Specimens with a mass of less than 100 mg were diluted with 0.5 mL; specimens with a mass of greater than 100 mg were diluted with 1.0 mL. The samples were then homogenized using the MagNA Lyser bead homogenizer for two 30 second pulses at 2000 rpm and then vortexed for an additional 30 seconds. The samples were pelleted by centrifugation at 12000 rcf for 5 minutes. 50 mg of extract were utilized for each specimen, and combined the fecal extract and D2O were a total volume of 630 µL. 70 µL of Chenomx ISTD solution into all tubes. Chenomx ISTD (Chenomx, Edmonton, Alberta, Canada) contains 5mM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS, chemical shift indicator), 100 mM imidazole (pH indicator), and 0.2% NaN3 (to inhibit bacterial growth) in D2O. 600 µL was transferred into each NMR tube for analysis. |
| Sampleprep Protocol Filename: | RTI_Food_Allergy_Envirnomental_Metabolomics_Procedure.docx |
