Summary of Study ST000237

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000190. The data can be accessed directly via it's Project DOI: 10.21228/M8Q30F This work is supported by NIH grant, U2C- DK119886.


Show all samples  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files
Study IDST000237
Study TitleQuick Comparison of Serum Metabolites in SD Rats of Different Sex by Untargeted UPLC-TOFMS and In-house Software Platform
Study TypeBiomarker Discovery
Study SummaryMale (n=8) and female (n=8) SD rats weighing between 220 and 250g were used. On the morning of sample collection day, each rat was deprived of food and put in metabolic cage for 24h urine collection. Then a blood sample (3-5ml) was collected from the aorta of the rat under anesthesia and centrifuged to obtain serum. All urine and serum samples were frozen at -80°C prior to analysis.
Beijing Institute of Radiation Medicine
DepartmentDepartment of Pharmacology and Toxicology
Last NameLiang
First NameQiande
Address27 Taiping Road, Beijing, P.R.China
Submit Date2015-08-09
Num Groups2
Total Subjects16
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2015-09-09
Release Version1
Qiande Liang Qiande Liang application/zip

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:SP000261
Sampleprep Summary:Amygdalin was dissolved in acetonitrile-water(1:1) to form 0.1mg/ml solution. Serum samples were unfrozen at room temperature. 500?l of serum sample was mixed with 20?l Amygdalin solution and 100?l 20% Trichloroacetic acid(TCA) solution. After centrifugation at about 14000rpm and -4?, the supernatant was injected into UPLC-TOF MS system.
Sampleprep Protocol Filename:2_PRE_PROT.pdf
Processing Method:Precipitation
Processing Storage Conditions:Precipitation at room tempreture; centrifugation at -4?
Extraction Method:Precipitation by TCA
Extract Concentration Dilution:500?l of serum/620?l
Extract Storage:4? after centrifugation and during analysis
Sample Spiking:20?l Amygdalin solution/620?l