Summary of Study ST000305
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000245. The data can be accessed directly via it's Project DOI: 10.21228/M8401H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000305 |
| Study Title | Pig Athersclerosis Model |
| Study Type | Metabolomics |
| Study Summary | Insulin-resistant subjects develop more severe and diffuse coronary artery atherosclerosis than insulin sensitive control but the mechanisms that mediate the atherosclerosis phenotype are unknown. The objective of this study is to investigate whether the severity of atherosclerosis is associated not only with lipoprotein concentrations, weight, blood pressure, biomarkers of inflammation and IR in an animal model but also changes in parameters that measure protein glycation. The experimental approach was to study normocholestrolemic pigs fed a high fat diet that also contained increased NaCl. The choice of pigs was driven by the fact that, like humans, they develop coronary artery and aortic atherosclerosis and insulin resistance. In addition, pigs have been used in many studies to define the mechanisms that mediate increased atherosclerosis in diabetes. |
| Institute | University of North Carolina |
| Department | Systems and Translational Sciences |
| Laboratory | Sumner Lab |
| Last Name | Sumner |
| First Name | Susan |
| Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
| susan_sumner @unc.edu | |
| Phone | 704-250-5066 |
| Submit Date | 2015-12-31 |
| Total Subjects | 90 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2016-12-31 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP000333 |
| Sampleprep Summary: | Serum samples were transferred to labeled tubes. A total of 112 study samples were thawed on ice for sample preparation 350 uL of serum sample were thawed and transferred to labeled tubes on ice where they were prepared and eventually mixed with 700uL master mix containing 630 uL of phosphate buffer and 70 uL of Chenomx ISTD (5mM DSS, 6mM Imidazole, and 0.02% NaN3). Analytical quality control (QC) phenotypic pooled samples were generated by transferring a 75 µL of each sample of each respective phenotypical experimental sample into different 15 mL tubes. Whole study (total) pools were generated by transferring 200 uL of serum from each Pool sample into a 15 mL tube. The tubes were vortexed for 4 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 min. A 600 µl aliquot of the supernatant was transferred into pre-labeled 5mm (4 inch) NMR tubes for data acquisition on a 700 MHz spectrometer.. |
| Sampleprep Protocol Filename: | Pig_Athersclerosis_Model_Procedures.docx |
