Summary of Study ST000361
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000287. The data can be accessed directly via it's Project DOI: 10.21228/M8Z310 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000361 |
Study Title | Characterization and Plasticity of the Metabolome in Peripheral Cells in Bipolar I Disorder |
Study Type | Broad Spectrum LCMS |
Study Summary | Patients with Bipolar I Disorder (BPI) and matched non-affected controls were recruited. Males and females from all races and ethnicities between the ages of 19-65 participated in the research. Skin biopsies were obtained and fibroblasts were isolated from each of these biopsies. A total of 1 x 〖10〗^7 fibroblasts cells were used for metabolomics. Cell pellets were flash frozen in liquid nitrogen and shipped on dry ice to the NIH Eastern Regional Comprehensive Metabolomic Resource Core at RTI International in North Carolina. Metabolomics will be determined using a broad spectrum metabolomics protocol by RTI |
Institute | University of North Carolina |
Department | Discovery Sciences |
Laboratory | Sumner Lab |
Last Name | Sumner |
First Name | Susan |
Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
susan_sumner @unc.edu | |
Phone | 704-250-5066 |
Submit Date | 2016-03-05 |
Num Groups | 2 |
Total Subjects | 18 |
Num Males | 7 |
Num Females | 11 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Waters) |
Analysis Type Detail | LC-MS |
Release Date | 2017-03-03 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000389 |
Sampleprep Summary: | Whole study pooled QC samples were created by combining 175 µL aliquot from each of the study samples into a 20 mL scintillation vial. The QC pooled sample was then vortexed for 30 seconds on an analog vortex mixer. Aliquots, 300 µL, were transferred to five new pre-labeled 2.0 mL LoBind Eppendorf tubes and stored at -80 °C for one hour. Next, the supernatants of the study and whole study pooled QC samples, generated above, were dried overnight using a lyophilizer. The residue was reconstituted in 75 µL of reconstitution buffer ( 95:5 Water:Methanol, v/v) and mixed on a multiple tube vortexer for five minutes at 5,000 rpm. Then, the samples were centrifuged at room temperature at 16,000 rcf for four minutes, and the supernatants were transferred to clean pre-labeled autosampler vials for data acquisition. |