Summary of Study ST000406

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000318. The data can be accessed directly via it's Project DOI: 10.21228/M8C59F This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000406
Study Title	Noninvasive Recognition and Biomarkers of Early Allergic Asthma in Cats using Multivariate Statistics of NMR Spectra of Exhaled Breath Condensate
Study Type	Statistical Analysis of NMR spectra of EBC samples
Study Summary	Asthma is prevalent in children and cats, and needs means of noninvasive diagnosis. We sought to distinguish noninvasively the differences in 53 cats before and soon after induction of allergic asthma, using NMR spectra of exhaled breath condensate (EBC). Statistical pattern recognition was improved by preprocessing the spectra with glog transformation. Classification of the 106 preprocessed spectra by principal component analysis, partial least squares with discriminant analysis (PLS-DA), and multi-level PLS-DA appears to be impaired by variances unrelated to eosinophilic asthma. By subtracting out confounding variances, orthogonal signal correction (OSC) PLS-DA greatly improved the separation of the healthy and early asthmatic states, attaining 94% specificity and 71% sensitivity in predictions. OSC-PLS-DA results highlight the elevation of acetone in two-thirds of the cats with early asthma. Asthma also decreased at least a dozen compounds, especially carboxylic acids such as short chain fatty acids and amino acids. These trends suggest that a majority of the cats with allergic asthma underwent alteration of metabolic fluxes through pyruvate and acetyl-CoA to promote ketosis. The noninvasive detection of early experimental asthma, its biomarkers in EBC, and metabolic rerouting invite further investigation of the diagnostic potential in humans.
Institute	University of Missouri-Columbia
Department	Department of Biochemistry, Department of Veterinary Medicine and Surgery
Laboratory	Van Doren Lab & Reinero Lab
Last Name	Van Doren
First Name	Steven
Address	117 Schweitzer Hall, Biochemistry Department, University of Missouri-Columbia, Columbia, MO 65211
Email	VanDorenS@missouri.edu
Phone	5738846405
Submit Date	2016-04-13
Num Groups	2
Total Subjects	53
Analysis Type Detail	NMR
Release Date	2016-06-18
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000434
Sampleprep Summary:	In all cats, Exhaled breath condensate (EBC)and Bronchoalveolar lavage fluid (BALF) and were collected before (day 0) and after (week 6) sensitization to BGA. The EBC was collected non-invasively just prior to BALF collection by placing cats in a 25 L plexiglass chamber for 20-30 min according to a previously published design with modifications [29]. BALF was collected in a blind fashion under anesthesia using an 8 French red rubber catheter passed through an endotracheal tube according to a previously described protocol [30]. The percentage of eosinophils in each BALF modified Wright’s stained cytospin was determined by counting 200 nucleated cells, with an asthmatic phenotype defined as >17% eosinophils.The EBC and the supernatant of centrifuged BALF remaining were promptly stored at -80 °C until further analysis. NMR spectra of the EBC were measured for use in training and evaluating statistical approaches and biomarkers.

Sampleprep ID:

SP000434

Sampleprep Summary:

In all cats, Exhaled breath condensate (EBC)and Bronchoalveolar lavage fluid (BALF) and were collected before (day 0) and after (week 6) sensitization to BGA. The EBC was collected non-invasively just prior to BALF collection by placing cats in a 25 L plexiglass chamber for 20-30 min according to a previously published design with modifications [29]. BALF was collected in a blind fashion under anesthesia using an 8 French red rubber catheter passed through an endotracheal tube according to a previously described protocol [30]. The percentage of eosinophils in each BALF modified Wright’s stained cytospin was determined by counting 200 nucleated cells, with an asthmatic phenotype defined as >17% eosinophils.The EBC and the supernatant of centrifuged BALF remaining were promptly stored at -80 °C until further analysis. NMR spectra of the EBC were measured for use in training and evaluating statistical approaches and biomarkers.