Summary of Study ST000406
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000318. The data can be accessed directly via it's Project DOI: 10.21228/M8C59F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000406 |
| Study Title | Noninvasive Recognition and Biomarkers of Early Allergic Asthma in Cats using Multivariate Statistics of NMR Spectra of Exhaled Breath Condensate |
| Study Type | Statistical Analysis of NMR spectra of EBC samples |
| Study Summary | Asthma is prevalent in children and cats, and needs means of noninvasive diagnosis. We sought to distinguish noninvasively the differences in 53 cats before and soon after induction of allergic asthma, using NMR spectra of exhaled breath condensate (EBC). Statistical pattern recognition was improved by preprocessing the spectra with glog transformation. Classification of the 106 preprocessed spectra by principal component analysis, partial least squares with discriminant analysis (PLS-DA), and multi-level PLS-DA appears to be impaired by variances unrelated to eosinophilic asthma. By subtracting out confounding variances, orthogonal signal correction (OSC) PLS-DA greatly improved the separation of the healthy and early asthmatic states, attaining 94% specificity and 71% sensitivity in predictions. OSC-PLS-DA results highlight the elevation of acetone in two-thirds of the cats with early asthma. Asthma also decreased at least a dozen compounds, especially carboxylic acids such as short chain fatty acids and amino acids. These trends suggest that a majority of the cats with allergic asthma underwent alteration of metabolic fluxes through pyruvate and acetyl-CoA to promote ketosis. The noninvasive detection of early experimental asthma, its biomarkers in EBC, and metabolic rerouting invite further investigation of the diagnostic potential in humans. |
| Institute | University of Missouri-Columbia |
| Department | Department of Biochemistry, Department of Veterinary Medicine and Surgery |
| Laboratory | Van Doren Lab & Reinero Lab |
| Last Name | Van Doren |
| First Name | Steven |
| Address | 117 Schweitzer Hall, Biochemistry Department, University of Missouri-Columbia, Columbia, MO 65211 |
| VanDorenS@missouri.edu | |
| Phone | 5738846405 |
| Submit Date | 2016-04-13 |
| Num Groups | 2 |
| Total Subjects | 53 |
| Analysis Type Detail | NMR |
| Release Date | 2016-06-18 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR000441 |
| Treatment Summary: | The care of the 53 cats in the study followed the NIH Guide for the Care and Use of Laboratory Animals. The University of Missouri Animal Care and Use Committee approved the study design (ACUC protocols #6912 and 7891). In order to induce allergic asthma, on day 0, cats <1 year of age were administered 12 µg of Bermuda grass allergen (BGA) in 10 mg of alum and 100 ng Bordetella pertussis toxin (both subcutaneously). On day 14, intranasal BGA (75 µg of BGA in 200 µL of phosphate-buffered saline) was given; and on day 21, 12 µg of BGA in 10 mg alum was injected subcutaneously. On day 28, the formation of wheals on an intradermal test confirmed sensitization to BGA. For the next 2 weeks, intensive aerosol challenges were administered to all cats with BGA (500 µg of BGA in 4 mL of phosphate-buffered saline) over 10 min in awake and unrestrained cats in a sealed plastic chamber. A nebulizer (Acorn nebulizer, model 646, Devilbis Health Care, Somerset, PA) delivered an aerosol of the BGA solution at an air flow rate of 9.3 l /min. An air compressor (Easy Air 15, Precision Medical, Inc., Northampton, PA) supplied the compressed air flow at a pressure of 2.93 kg/cm2. At week 6, samples were collected 24 h after challenge with BGA aerosol (see below). |
| Treatment Compound: | Bermuda grass allergen (BGA) |
