Summary of Study ST000585

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000428. The data can be accessed directly via it's Project DOI: 10.21228/M8QW3H This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST000585
Study Title	Metabolomic profiling of follicular fluid from patients with infertility-related deep endometriosis.
Study Summary	the metaboloc qualitative profiling was performed by LC-MS in follicular fluid samples of controls and endometriosis patients undergoing in vitro fertilization treatment
Institute	Sao Paulo Federal University
Last Name	Cordeiro
First Name	Fernanda
Address	Rua Embau, 231, Vila Clementino
Email	fernandabertuccez85@gmail.com
Phone	11996667402
Submit Date	2017-04-06
Num Groups	2
Total Subjects	40
Num Females	40
Raw Data Available	Yes
Raw Data File Type(s)	raw(Waters)
Analysis Type Detail	LC-MS
Release Date	2017-07-10
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000615
Sampleprep Summary:	The FF samples were centrifuged at 800 × g for 10 minutes to separate the fluid from follicular cells and the samples were maintained frozen in -20 °C until the metabolites extraction. The Bligh & Dyer protocol (1959) was applied for proteins removal and was performed as herein: 50 µL of sample was placed in a microtube, followed by addition of 50 µL of milli-Q water, 125 µL of chloroform (CHCl3 - Merck Millipore, MA, EUA) and 250 µL of methanol (MeOH - Merck Millipore - Massachusetts, EUA). The mixture was vortexed for 30 seconds. Next, polar and apolar phases were separated by the addition of 100 µL of milli-Q water and 125 µL of CHCl3 (Merck Millipore, MA EUA). Both polar and apolar phases were collected and transferred to a new microtube. The final content was dried by using a speedvac (CentriVap Cold Trap, Labconco, MO, USA). Prior to the LC-MS analysis, the metabolites were recovered by using 100 µL of acetonitrile and water (ACN:H2O 1:1, v:v - Merk Millipore, MA, USA) and filtered in 0.22 μm PVDF filters (Merck Millipore, MA, EUA).

Sampleprep ID:

SP000615

Sampleprep Summary:

The FF samples were centrifuged at 800 × g for 10 minutes to separate the fluid from follicular cells and the samples were maintained frozen in -20 °C until the metabolites extraction. The Bligh & Dyer protocol (1959) was applied for proteins removal and was performed as herein: 50 µL of sample was placed in a microtube, followed by addition of 50 µL of milli-Q water, 125 µL of chloroform (CHCl3 - Merck Millipore, MA, EUA) and 250 µL of methanol (MeOH - Merck Millipore - Massachusetts, EUA). The mixture was vortexed for 30 seconds. Next, polar and apolar phases were separated by the addition of 100 µL of milli-Q water and 125 µL of CHCl3 (Merck Millipore, MA EUA). Both polar and apolar phases were collected and transferred to a new microtube. The final content was dried by using a speedvac (CentriVap Cold Trap, Labconco, MO, USA). Prior to the LC-MS analysis, the metabolites were recovered by using 100 µL of acetonitrile and water (ACN:H2O 1:1, v:v - Merk Millipore, MA, USA) and filtered in 0.22 μm PVDF filters (Merck Millipore, MA, EUA).