Summary of Study ST001021

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000683. The data can be accessed directly via it's Project DOI: 10.21228/M8D11B This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST001021
Study Title	Characterization of metabolomics profile changes during development of post-traumatic epilepsy in Rat Cerebrospinal Fluid (part-II)
Study Summary	Characterize the metabolomics profile changes during progression of the transition from traumatic brain injury (TBI) to post-traumatic epilepsy (PTE). To do so, three experiments will be performed. PTE animal model will be developed using ferrous chloride injections. Metabolomics profile changes will be obtained before TBI, after TBI, and after PTE development These temporal changes in metabolomics profile during the course of PTE development will be collected. We will also collect cerebrospinal fluid (CSF) at each time point. In addition, we will collect the brain tissue from the center of injury, around the injury, and from the non-injured area for mass spectrometry. In this study, Rat CSF is analyzed at end of study.
Institute	Mayo Clinic
Last Name	Su-youne
First Name	Chang
Address	200 1st Street SW Rochester, MN 55905, USA
Email	Chang.SuYoune@mayo.edu
Phone	1-507-293-0511
Submit Date	2018-07-17
Analysis Type Detail	LC-MS
Release Date	2020-07-15
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001067
Sampleprep Summary:	large scale profiling of rat cerebral spinal fluid The brain tissue and CSF will be collected for mass spectrometry. To prepare samples, proteins will be removed from collected dialysates by adding cold methanol:water (8:1, v/v) mixture containing 5.0 μg internal standard (IS), myristic-d27 acid, at ambient temperature. Samples will be vortexed for 1 min, incubated on ice for 15 min, and then centrifuged. The supernatant will be completely dried in a SpeedVac, and the lyophilized sample will be subsequently methoxiaminated using 20 μl of a 20 mg/ml solution of methoxyamine hydrochloride in pyridine at 30°C for 90 min and derivatized using 80 μL of N-methyl-N-trimethylsilyltrifluoroacetamide with 1% trimethylchloro-silane (MSTFA + 1% TMCS, Pierce) at 37°C for 30 min.

Sampleprep ID:

SP001067

Sampleprep Summary:

large scale profiling of rat cerebral spinal fluid The brain tissue and CSF will be collected for mass spectrometry. To prepare samples, proteins will be removed from collected dialysates by adding cold methanol:water (8:1, v/v) mixture containing 5.0 μg internal standard (IS), myristic-d27 acid, at ambient temperature. Samples will be vortexed for 1 min, incubated on ice for 15 min, and then centrifuged. The supernatant will be completely dried in a SpeedVac, and the lyophilized sample will be subsequently methoxiaminated using 20 μl of a 20 mg/ml solution of methoxyamine hydrochloride in pyridine at 30°C for 90 min and derivatized using 80 μL of N-methyl-N-trimethylsilyltrifluoroacetamide with 1% trimethylchloro-silane (MSTFA + 1% TMCS, Pierce) at 37°C for 30 min.