Summary of Study ST001268
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000853. The data can be accessed directly via it's Project DOI: 10.21228/M8F108 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001268 |
| Study Title | Metabolome data to annotate metabolite origins |
| Study Summary | Plasma metabolomics from HIV subjects and controls was incorporated with microbiome data to develop Annotation of Metabolite Origins via Networks (AMON). |
| Institute | University of Colorado Denver |
| Department | Anschutz Medical Campus |
| Last Name | Reisdorph |
| First Name | Nichole |
| Address | 12850 East Montview Blvd, Aurora, CO, 80045, USA |
| nichole.reisdorph@ucdenver.edu | |
| Phone | 3037249234 |
| Submit Date | 2019-10-22 |
| Total Subjects | 63 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-10-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001344 |
| Sampleprep Summary: | 100 µL of plasma spiked with internal standards underwent a protein crash with 400 µL ice cold methanol. The supernatant was dried under nitrogen. Methyl tert-butyl ether (MTBE) and water were added to extract the hydrophobic and hydrophilic compounds, respectively. The upper hydrophobic layer was transferred to a new tube and the lower hydrophilic layer was re-extracted with MTBE. The upper hydrophobic layer was combined, dried under nitrogen and reconstituted in 200 µL of methanol. The hydrophilic layer was dried under nitrogen, underwent a second protein crash with water and ice-cold methanol (1:4 water-methanol). The supernatant was removed, dried by SpeedVac at 45 °C and reconstituted in 100 µL of 5% acetonitrile in water. Both fractions were stored at -80 °C until LCMS analysis. |
| Processing Storage Conditions: | On ice |
