Summary of Study ST001294
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000876. The data can be accessed directly via it's Project DOI: 10.21228/M8FX1M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001294 |
| Study Title | Estimating Platelet Mitochondrial Function in Patients with Sepsis - Platelet NMRs (part-I) |
| Study Type | single timepoint |
| Study Summary | Relationships between platelet mitochondrial oxygen consumption rates (mOCR) and metabolites in platelets as measured by quantitative 1H-NMR metabolomics. Samples collected in ED at a single timepoint. WB and platelets isolated from the same blood samples. Comparison of mitochondrial function and metabolomics in patients with sepsis and non-sepsis ED patients |
| Institute | University of Michigan; University of Mississippi; University of Minnesota |
| Department | Clinical Pharmacy (UMich); Emergency Medicine (UMiss) |
| Laboratory | Stringer NMR Metabolomics Laboratory |
| Last Name | McHugh |
| First Name | Cora |
| Address | 428 Church St, Ann Arbor, MI, 48103, USA |
| NMRmetabolomics@umich.edu | |
| Phone | 7343530164 |
| Submit Date | 2019-12-13 |
| Num Groups | 2 |
| Total Subjects | 23 |
| Num Males | 12 |
| Num Females | 11 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2020-05-26 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001376 |
| Sampleprep Summary: | Methanol chlorform extraction of platelet pellet after 2 freeze-thaw cycles in the presence of methanol. |
| Sampleprep Protocol ID: | Platelet Pellet MeOHCHCl3 Extraction |
| Sampleprep Protocol Filename: | 233.2.1_-_HAPS_Platelet_Pellet_MeOHCHCl3_Extraction |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Methanol chloroform extraction |
| Extract Enrichment: | Lyophilization |
| Extract Storage: | -80℃ |
| Sample Resuspension: | 500uL 50mM sodium phosphate buffer in D2O |
| Sample Spiking: | 4.99 mM DSS internal standard |
