Summary of Study ST001379
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000916. The data can be accessed directly via it's Project DOI: 10.21228/M8912S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001379 |
| Study Title | 2,3,7,8 -Tetrachlorodibenzo-p-dioxin Mediated Effects on Hepatic Coenzyme A (CoA) and Acetyl-CoA Levels (part-II) |
| Study Type | Dioxin |
| Study Summary | Experiment to study TCDD-elicited effects on hepatic Coenzyme A (CoA) and Acetyl-CoA levels in liver of male mice treated with sesame oil vehicle or 0.01-30 ug/kg TCDD every 4 days for 28 days. |
| Institute | Michigan State University |
| Department | Biochemistry and Molecular Biology |
| Laboratory | Zacharewski Lab |
| Last Name | Fling |
| First Name | Russell |
| Address | 1129 Farm Ln, East Lansing, MI, USA |
| flingrus@msu.edu | |
| Phone | (517) 884-2054 |
| Submit Date | 2020-04-01 |
| Num Groups | 9 |
| Total Subjects | 80 |
| Num Males | 29 |
| Study Comments | Fax: (517) 353-9334; ORCID: 0000-0002-6822-4962; ROLE: Graduate Student; Pubmed ID: -;Publication DOI: -; |
| Publications | - |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-08-02 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001461 |
| Sampleprep Summary: | Frozen liver tissue was homegenized in 4.6 ml h20:methanol (5:3) mixture and homegenized, 2.9 ml chloroform was added,vortexed and spun down at 5,000G. Top layer was removed and blown down with nitrogen and reconstituted in 400 l 10mM tetrabutylammonium (TBA)+10mM acetate (pH 5). A further 1:16 dilution was performed for analysis of acetyl-CoA. Coenzyme A (CoA) was measured by standard addition method by diluting with TBA containing CoA standard resulting in 1 uM CoA standard addition. 1 uM CoA standard addition samples are denoted in the subject sample factor section. |
| Processing Method: | Liquid extraction |
| Processing Storage Conditions: | -80℃ |
