Summary of study ST001859

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001172. The data can be accessed directly via it's Project DOI: 10.21228/M86T2S This work is supported by NIH grant, U2C- DK119886.

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Study IDST001859
Study TitledTor affects the fat body lipidome via Nep1r1, Ctdnep1 and Lipin
Study SummaryQuantitative MS analysis was performed on ten 4 day-old Drosophila larval fat bodies homogenized in 50µl D-PBS (Dulbecco’s Phosphate Buffered Saline without Mg2+ and Ca2+) by Lipotype using previously described methods (Grillet et al, 2016).
Institute
VIB KULeuven
DepartmentDept. of Neurosciences, KU Leuven, 3000 Leuven, Belgium
Last NameJacquemyn
First NameJulie
AddressON 4, 6e verd Campus Gasthuisberg, Herestraat 49, bus 602, Leuven, NA, 3000, Belgium
Emailjulie.jacquemyn@kuleuven.be
Phone0032479570951
Submit Date2021-06-22
Analysis Type DetailLC-MS
Release Date2021-07-18
Release Version1
Julie Jacquemyn Julie Jacquemyn
https://dx.doi.org/10.21228/M86T2S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001942
Sampleprep Summary:Lipids from fat body tissues of indicated genotypes were extracted using chloroform and methanol (1). Samples were spiked with lipid class-specific internal standards prior to extraction. After drying and resuspending in MS acquisition mixture, lipid extracts were subjected to mass spectrometric analysis. Sample preparations were done by Lipotype. (1) Sampaio, J. L. et al. Membrane lipidome of an epithelial cell line. Proceedings of the National Academy of Sciences 108, 1903–1907 (2011).
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