Summary of Study ST001872
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001181. The data can be accessed directly via it's Project DOI: 10.21228/M82418 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001872 |
Study Title | Untargeted LC-MS metabolomics analysis of cecal content of mice treated with TCDD vs. vehicle control (part II) |
Study Type | Untargeted metabolomics |
Study Summary | Six-week-old female wildtype (WT) C57BL/6 mice were administered a single 100µl intraperitoneal injection containing sterile corn oil (VEH group) or an intraperitoneal injection of 10µg/kg TCDD suspended within sterile corn oil (TCDD group). At the 72h time point following TCDD or VEH exposure, the mice were humanely euthanized by an overdose of inhaled isoflurane. During necropsy, cecal content and blood serum samples were collected for untargeted metabolomics profiling. |
Institute | University of South Carolina School of Medicine |
Department | Department of Pathology |
Laboratory | (On behalf of) Mitzi Nagarkatti Lab |
Last Name | Lai |
First Name | Yunjia |
Address | 135 Dauer Drive, Chapel Hill, NC 27599 |
yunjia.lai@outlook.com | |
Phone | 919-480-5489 |
Submit Date | 2021-07-18 |
Num Groups | 2 |
Total Subjects | 20 |
Num Females | 20 |
Publications | Chemosphere |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2021-08-30 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001955 |
Sampleprep Summary: | Cecal content and serum samples were processed according to previously described methods for the metabolomic profiling using liquid chromatography-mass spectrometry (LC-MS) with slight modifications. For cecal content, ~25 mg was aliquoted into 1.5-mL Eppendorf tubes (Hamburg, Germany) containing ~20 mg acid washed glass beads (Sigma-Aldrich, St. Louis, MO), extracted into ice-cold MeOH:water (1:1, v/v) on a Qiagen TissueLyzer at 50 Hz for 10 min (Hilden, Germany), and centrifuged at 12,000 rpm for 10 min. For blood sera, 20 µL aliquots were extracted by adding 180 µL cold MeOH, briefly vortexed, and incubated at -20 ºC for 30 min for protein precipitation. The supernatants of both matrices were dried in a CentriVap vacuum concentrator (Labconco, MO) and resuspended in ACN:water (2:98, v/v) upon analysis. |