Summary of Study ST001880
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001186. The data can be accessed directly via it's Project DOI: 10.21228/M8DD61 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001880 |
| Study Title | NMR Predator Cues Target Signaling Pathways in Toxic Algal Metabolome (Polar metabolites) |
| Study Type | 1H NMR Metabolomics to elucidate signaling pathway |
| Study Summary | Metabolomics investigation of the phytoplankton Alexandrium minutum with and without copepod cues in order to explore cell signaling involved in toxin induction. |
| Institute | Georgia Institute of Technology |
| Department | School of Biological Sciences, School of Chemistry and Biochemistry, Center for Microbial Dynamics and Infection, Parker H. Petit Institute for Bioengineering and Bioscience |
| Laboratory | Kubanek Lab |
| Last Name | Brown |
| First Name | Emily |
| Address | 950 Atlantic Dr Atlanta, Georgia USA 30332 |
| julia.kubanek@biosci.gatech.edu | |
| Phone | 404-894-8424 |
| Submit Date | 2021-07-15 |
| Num Groups | 2 |
| Total Subjects | 40 |
| Study Comments | Part 1 of 3. This part includes NMR analysis of polar metabolites using oPLSDA. Parts 2 and 3 inlcude NMR analysis of nonpolar metabolites and the corresponding mass spectrometry metabolomics for both polar and non-polar metabolites. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2021-07-27 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001966 |
| Sampleprep Summary: | Polar extracts were dissolved at a concentration equivalent to 1.70 x 10^7 A. minutum cells mL-1 in 90:10 H2O/D2O (99.96% atom D2O; Cambridge Isotope Labs) with 0.2 mM phosphate buffer (pH 7.4) and 0.25 mM 3 (trimethylsilyl)propionic 2,2,3,3d 4 acid (TMSP), as internal standard, for 1H NMR spectroscopy analysis. The media blank extract was prepared in the smallest volume of solvent possible (175 µL). Particulates were excluded by briefly centrifuging the samples and then carefully transferring the extracts into 3 mm NMR tubes. The extracts were analyzed using a Bruker Avance IIIHD 800 MHz NMR spectrometer equipped with a 3 mm triple resonance broadband cryoprobe. Spectra for the polar extracts were acquired using a 1D water presaturation (Bruker zgpr pulse sequence) with a spectral width of 12 kHz centered at 3758.36 Hz, with a relaxation delay 3 s, over 480 scans. |
| Sampleprep Protocol Filename: | Predator Cues Target Signaling Pathways in Toxic Algal Metabolome_protocol.pdf |
| Extract Storage: | -80℃ |
