Summary of Study ST002198
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001401. The data can be accessed directly via it's Project DOI: 10.21228/M8MT48 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002198 |
| Study Title | Untargeted metabolomics of Pinus pinaster needles under heat and drought stress |
| Study Type | Untargeted MS-based metabolomics |
| Study Summary | Current projections for global climate change predict an increase in the intensity and frequency of heat waves and droughts. The improvement in our understanding of the mechanisms of how trees precisely can predict environmental threats and cope with these stresses benefits our natural selection or genetic improvement to the maintenance of forest sustainability. In this work, we investigate the metabolic changes in heat and drought combined stress in Pinus pinaster plantlets. Maritime pine is a coniferous tree with native populations distributed across the European Atlantic and Mediterranean basins and the north of Africa ranging from cool moist to warm dry climates. This species shows high plasticity and a contrasting adaptive capacity and resilience. This plasticity in the response to stress exposure may be associated with a differential ability to modulate their secondary metabolism. For this reason, the current study aims to investigate the gradual and synergetic metabolomic response using liquid chromatography coupled to mass spectrometry (LC-MS) based on untargeted metabolomic profiling of four stress levels. These metabolic profiles were supported by physiological and biochemical determinations. Our results showed that the metabolic profiles induced by low-stress exposition represent an adaptive conditioning mode with metabolome changes that help seedlings to cope with upcoming stress. The metabolism pathways involved in this response were mainly included in amino acid metabolism and carbohydrate metabolism leading to an enhanced accumulation of phenolics, flavonoids, and terpenoids. However, when the plantlets were exposed to higher-stress exposition, the secondary metabolites that starred the response are more complex and decorated, such as alkaloids, lignans, and glycosyloxyflavones. Those changes could help to maintain homeostasis and control the response magnitude on establishing and facilitating the plantlets’ survival. Overall, our findings provide new insights into the responsive mechanisms of the maritime pine under heat and drought stress in terms of metabolic profiles. |
| Institute | Universidad de Oviedo |
| Department | Department of Organisms and Systems Biology |
| Laboratory | Plant Physiology |
| Last Name | López Hidalgo |
| First Name | Cristina |
| Address | C/ Catedrático Rodrigo Uría s/n Oviedo 33071 |
| lopezhcristina@uniovi.es | |
| Phone | 985104774 |
| Submit Date | 2022-06-16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-07-14 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002290 |
| Sampleprep Summary: | Metabolites were extracted from 20 mg (lyophilized weight) of needles. Metabolite extraction was performed according to Valledor et al., (2014). Briefly, 600 µL of cold (4 ºC) metabolite extraction solution (methanol: chloroform: H2O (2.5:1:0.5) was added to each tube and strongly vortexed. Then, the tubes were incubated in a cold ultrasound bath for 10 min. Later, the tubes were centrifuged at 20.000 x g for 6 min at 4 °C. The supernatant containing metabolites from each tube was transferred to a new tube containing 300 µL of chloroform: water (1:1) to allow phase separation. Six hundred µL of cold (4 ºC) metabolite extraction solution were added to the remaining pellets, and vortexing, ultrasound bath, and centrifugation were repeated. The new supernatant was transferred to the previous tube that contained the phase separation solution and the old supernatant. These tubes were vortexed and then centrifuged at 15.000 x g for 5 min at 4 °C. After centrifugation, two layers are formed; the upper-aqueous layer (methanol: water) containing the polar metabolites was transferred to a separate microcentrifuge tube and then cleaned from non-polar metabolites adding 300 µL of cold (4 ºC) chloroform: water (1:1), vortexed, and centrifuged at 15.000 x g for 4 min at 4 °C. The new upper phase was transferred to a new tube. The polar extract was dried using a speedvac at 25 °C. |
| Processing Method: | Methanol:Chloroform:Water |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Methanol:Chloroform:Water |
| Extract Enrichment: | Polar metabolites |
| Extract Cleanup: | Centrifugation |
| Extract Storage: | -80℃ |
| Sample Resuspension: | Methanol |
| Sample Derivatization: | NO |
| Sample Spiking: | NO |
